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Bt-BADH-GA20ox-rolB多基因在转化烟草中的表达分析

孙立洋, 李静, 袁虎威, 姚景涵, 李伟, 陈晓阳

孙立洋, 李静, 袁虎威, 姚景涵, 李伟, 陈晓阳. Bt-BADH-GA20ox-rolB多基因在转化烟草中的表达分析[J]. 北京林业大学学报, 2011, 33(5): 86-90.
引用本文: 孙立洋, 李静, 袁虎威, 姚景涵, 李伟, 陈晓阳. Bt-BADH-GA20ox-rolB多基因在转化烟草中的表达分析[J]. 北京林业大学学报, 2011, 33(5): 86-90.
SUN Li-yang, LI Jing, YUAN Hu-wei, YAO Jing-han1, LI Wei, CHEN Xiao-yang. Expression analysis of Bt-BADH-GA20ox-rolB multigenes in transgenic tobacco plants[J]. Journal of Beijing Forestry University, 2011, 33(5): 86-90.
Citation: SUN Li-yang, LI Jing, YUAN Hu-wei, YAO Jing-han1, LI Wei, CHEN Xiao-yang. Expression analysis of Bt-BADH-GA20ox-rolB multigenes in transgenic tobacco plants[J]. Journal of Beijing Forestry University, 2011, 33(5): 86-90.

Bt-BADH-GA20ox-rolB多基因在转化烟草中的表达分析

Expression analysis of Bt-BADH-GA20ox-rolB multigenes in transgenic tobacco plants

  • 摘要: 为探讨Bt-BADH-GA20ox-rolB多基因转化烟草中外源基因的表达情况及对转化植株的影响,对转多基因烟草及对照植株进行了生理生化试验,包括内源激素含量测定、耐盐性试验、Bt毒蛋白测定、移栽后植株生长的观测。结果表明:转基因植株生根能力强,根、茎、叶各部位内源激素(IAA、GA3)与对照差异明显;在低浓度NaCl下,转基因和对照植株生长差异不明显,当NaCl浓度达到300 mmol/L时,对照生长受到抑制,转基因植株生长正常;在转基因植株的茎、叶中均检测到高浓度的Bt毒蛋白,以叶片饲喂斜纹夜蛾幼虫明显抑制其发育。移栽后的转基因植株高生长更加明显,苗高和地茎分别为对照的1.61和1.37倍。
    Abstract: In order to discuss the exogenous genes expression and effect of Bt-BADH-GA20ox-rolB multigenes on transgenic plants, four transgenic tobacco lines and a control strain were taken as materials and the experiments included determination of endogenous hormones, salt tolerance test, Bt toxic protein measurement and growth observation. The results indicated that the rooting ability of transgenic plants was enhanced. Endogenous hormones(IAA, GA3)derived from roots, stems and leaves were significantly different from controls. At a low concentration of NaCl, there is no difference between transgenic plant and control, while at 300 mmol/L of NaCl the growth of control was restrained with and the transgenic plant was normal. The Bt toxic protein was detected at a high level in both stems and leaves of the transgenic plants. The development of prodenia was obviously inhibited by feeding the leaves of transgenic plants. The transgenic plants grew vigorously after being transplanted. The average height and basal stem of transgenic plants were 1.61 and 1.37 times higher than those of control respectively.
  • 期刊类型引用(6)

    1. 肖丹丹,张旭,任亚超,王桂英,左力辉,董研,杨敏生,王进茂. 3个抗虫耐盐基因载体对烟草的遗传转化及表达分析. 分子植物育种. 2017(01): 166-173 . 百度学术
    2. 任亚超,董研,张文林,杨敏生. 转Bt基因烟草中Cry1Ac基因的叠加效应. 农业生物技术学报. 2017(12): 1903-1917 . 百度学术
    3. 蒋玉蓉,袁俊杰,陈国林,祝水金. 转甜菜碱醛脱氢酶(BADH)基因棉花的获得及其耐盐性鉴定. 分子植物育种. 2015(01): 125-131 . 百度学术
    4. 赵先海,湛欣,邓小梅,袁金英,赵帅. 转MdSPDS1-AtCBF1双价基因烟草抗逆境研究. 中国农业大学学报. 2015(03): 46-50 . 百度学术
    5. 刘娇娇,王桂英,任亚超,董研,徐丽娜,梁海永. BtCry1Ac、BADH双价基因对烟草的遗传转化及表达检测. 河北林果研究. 2014(02): 169-174 . 百度学术
    6. 杜沙沙,张旭,王进茂,董研,杨敏生. 农杆菌介导BtCry1Ac、NTHK1双价基因转化烟草植株的研究. 河南农业科学. 2014(07): 31-37 . 百度学术

    其他类型引用(9)

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出版历程
  • 收稿日期:  1899-12-31
  • 修回日期:  1899-12-31
  • 发布日期:  2011-09-29

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