中国美国白蛾种群遗传多样性的AFLP分析
Genetic diversity of Hyphantria cunea populations in China by AFLP analysis
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摘要: 利用AFLP分子标记技术分析我国重大外来入侵害虫美国白蛾8个群体的遗传多样性及遗传分化。结果表明：5对AFLP引物共扩增出564个位点，其中多态位点为548个，多态位点百分率（P）为97.16%，Neis遗传多样性指数（h）为0291 0，在物种水平上美国白蛾具较高的遗传多样性；AMOVA显示美国白蛾有较高的遗传分化，群体间变异占31.05%，群体内变异占68.95%，遗传分化指数（Fst）为0.310 5；美国白蛾8个群体平均遗传相似度为0.892 7，平均遗传距离为0.114 7。聚类分析结果表明，沈阳、济南、烟台和寿光群体亲缘关系较近，天津、济宁群体聚为一类，而廊坊、北京群体分别独立分支。进而对美国白蛾遗传多样性与其适应机制和扩散传播模式之间的关系进行了讨论。Abstract: Amplified fragment length polymorphism（AFLP）makers were used to analyze the genetic diversity and genetic differentiation in eight populations of Hyphantria cunea. The results showed that five primers used in this study produced 548 polymorphic loci out of 564 loci totally. The percentage of polymorphic loci（P）was 97.16% and Neis gene diversity（h）was 0.291 0, indicating high genetic diversity in H. cunea. Analysis of molecular variance（AMOVA）revealed that a high proportion（68.95%）of the total genetic variation was partitioned among populations, suggesting significant genetic differentiations among the eight populations of H. cunea. Gene differentiation coefficient（Fst）among populations was 0.310 5. Average genetic similarity of the eight populations was 0.892 7, while average genetic distance was 0.114 7. Eight populations were clustered into four groups in unweighted pairgroup mean analysis（UPGMA）. The relationship between the genetic diversity and the mechanism of its environmental adaptation and population expansion was further discussed.
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