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    5种松毛虫翅面鳞片超微结构观察与比较

    Comparison among the ultrastructures and characteristics of wing scales in 5 species of pine caterpillar moths

    • 摘要: 利用扫描电子显微镜观察马尾松毛虫、油松毛虫、落叶松毛虫、赤松毛虫和思茅松毛虫的翅面鳞片超微结构,发现这5种松毛虫的鳞片分为基鳞与覆鳞2种类型,都属于镂空型鳞片,且为二型双层鳞片。对5种松毛虫的翅面鳞片的长度、宽度、脊间距、最宽处纵脊数进行了测量与比较,形态表征统计分析结果显示:1)鳞片的长宽与翅长不存在显著相关性;2)5种松毛虫的翅面鳞片不存在雌雄间差异,前后翅及翅正反面鳞片均无显著差异;3)种间鳞片宽度、脊间距无显著差异,而长度、最宽处纵脊数均存在显著差异。对翅面鳞片的形态表征进行系统聚类分析,结果显示,同一种松毛虫的不同地理种群之间鳞片大小相似性较高,即同种松毛虫的不同地理种群通常聚为一类。翅面鳞片的超微形态特征不宜单独用于种的鉴定,但可为亲缘关系的研究提供一定的参考。

       

      Abstract: Investigation about the ultrastructures of wing scales among 5 species, i.e., Dendrolimus punctata punctata, D. tabulieformis,D. superans, D. spectabilis and D. kikuchii kikuchii, by scanning electron microscopy(SEM) showed that there were ground scales and cover scales, and both of them were hollow scales and their type of covering was type2 bilayer scale covering.The length of scales(length),width of scales(width),distance between ridges on scales(ridgedistance),and the maximum number of the scales ridges(mixridgenumber) were characterized and compared among species. Results showed that there were no obvious correlations existing between the length and width of scales and the length of wings. There were no significant differences in scales between male and female moths, forewings and hind wings,upsidewings and undersidewings either. The discrepancies existing in the width and the ridgedistance of the scales among the five moth species were inconspicuous while the corresponding part of the length and the mixridgenumber of scales among species was striking. The characteristics of scales were analyzed by hierarchical cluster. The consequences show that the similarity of wing scales among different geographic populations of the same moth is higher,i.e.different geographic populations of the same species are usually clustered together.Although the ultrastructure and characteristics of wing scales could provide some evidence for the study of phylogeny,they should not be used alone for species identification.

       

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