高温诱导杜仲2n花粉最佳处理条件研究
The optimum conditions for inducing 2n pollen chromosome doubling by high temperature in Eucommia ulmoides
-
摘要: 在掌握杜仲小孢子母细胞减数分裂规律的基础上，采用“树木非离体枝芽加热处理装置”处理发育到一定程度的杜仲雄花芽，研究了小孢子母细胞减数分裂时期、处理温度以及持续时间等因素对杜仲2n花粉诱导比率的影响，探讨高温诱导杜仲2n花粉的适宜条件。结果表明：杜仲雄花芽的长度、直径以及花药伸出鳞片的长度与小孢子母细胞减数分裂进程有显著相关性，可以作为初步判别减数分裂进程的参照；引入“加权减数分裂时期”的概念更有利于解决各同步性较差的处理材料发育时期的确定问题，且有利于不同处理的数量化对比分析；施加高温处理诱导杜仲2n花粉时，花粉母细胞减数分裂时期、处理温度和持续处理时间对2n花粉诱导率有显著影响，高温诱导杜仲花粉染色体加倍的最佳处理时期是一个比较宽泛的时间过程，在小孢子母细胞减数分裂的双线期、终变期、中期Ⅰ、后期Ⅰ、中期Ⅱ施加高温处理，均可获得超过40%的2n花粉诱导率；杜仲雄花芽对高温处理相对不敏感，需要更高的处理温度和较长的持续处理时间。Abstract: Based on the rule of meiosis of microspore, the effects of high temperature, duration and meiotic stages on percentage of 2n pollen were determined by a “living tree hightemperature doubler” using Eucommia ulmoides as the experimental material. The optimum conditions of 2n pollen induction were discussed. The experimental results indicated that significant correlations were found between meiotic stages and mean length, and mean diameter of male buds, and mean exposure length of anther. Thus these morphological indexes could be considered as a marker to the process of meiosis. The concept of weighted mean stage was taken into determination of meiotic stages in asynchronous studied material, which helped compare the quantified differences derived from different treatment combinations. Significant effects on induction percentage of 2n pollen from meiotic stages, high temperature and duration were observed when 2n pollen was induced by high temperature. To some extent, the optimum stage for pollen chromosome doubling by high temperature was a extensive process of meiotic stages. Exceeding 40% of 2n pollen could be obtained by high temperature treatment during diplotene, diakinesis, metaphse Ⅰ, anaphase Ⅰ and metaphse Ⅱ, respectively. Sensitivity to high temperature for male buds was relatively low in E. ulmoides. Therefore, a higher temperature and longer duration are needed when 2n pollen is induced by high temperature treatment.
-
-
期刊类型引用(8)
1. 李亚鹏，孙宇涵，林华忠，方禄明，余小龙，翁建宇，张运根，李云. 杉木小孢子发生与雄球花发育相关性研究. 北京林业大学学报. 2023(01): 51-58 . 
本站查看
2. 刘春洋，彭朝凤，程世平，姚鹏强，耿喜宁，谢丽华. 高温诱导‘凤丹’牡丹2n雌配子创制三倍体. 园艺学报. 2023(07): 1455-1466 . 百度学术
3. 吴婷，贾瑞冬，杨树华，赵鑫，于晓南，国圆，葛红. 蝴蝶兰多倍体育种研究进展与展望. 园艺学报. 2022(02): 448-462 . 百度学术
4. 程世平，姚鹏强，耿喜宁，刘春洋，谢丽华. 高温诱导牡丹产生未减数花粉. 园艺学报. 2022(03): 581-589 . 百度学术
5. 买旖旎，李树战，索玉静，孙鹏，韩卫娟，刁松锋，王丽媛，李华威，傅建敏. 柿不同种质中的天然2n花粉得率的评价及诱导时期的判定. 中国农业大学学报. 2019(12): 44-52 . 百度学术
6. 田梦迪，李燕杰，张平冬，王健，郝静颐. 高温诱导银灰杨花粉染色体加倍创制杂种三倍体. 林业科学. 2018(03): 39-47 . 百度学术
7. 康向阳. 杜仲良种选育研究现状及展望. 北京林业大学学报. 2017(03): 1-6 . 本站查看
8. Jun Yang，Jun Lan，Pengqiang Yao，Zhen Huang，Xiangyang Kang. Comparative microsporogenesis and flower development in Eucalyptus urophylla × E. grandis. Journal of Forestry Research. 2016(02): 257-263 . 必应学术
其他类型引用(14)
计量
- 文章访问数: 1615
- HTML全文浏览量: 191
- PDF下载量: 77
- 被引次数: 22
下载:
