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钮世辉, 李伟, 陈晓阳. 赤霉素对根尖径向生长的调节作用研究[J]. 北京林业大学学报, 2013, 35(3): 71-76.
引用本文: 钮世辉, 李伟, 陈晓阳. 赤霉素对根尖径向生长的调节作用研究[J]. 北京林业大学学报, 2013, 35(3): 71-76.
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NIU Shi-hui, LI Wei, CHEN Xiao-yang. Negative regulation of gibberellin on root tip diameter.[J]. Journal of Beijing Forestry University, 2013, 35(3): 71-76.
Citation: NIU Shi-hui, LI Wei, CHEN Xiao-yang. Negative regulation of gibberellin on root tip diameter.[J]. Journal of Beijing Forestry University, 2013, 35(3): 71-76.
shu

赤霉素对根尖径向生长的调节作用研究

Negative regulation of gibberellin on root tip diameter.

    摘要
  • 摘要: 通过转基因与RNAi 基因沉默技术,获得了由35S 组成型启动子和TobRB7 根尖特异表达型启动子分别启动 表达的PtGA20ox、PtGA2ox1 烟草转基因株系和NtGA20ox RNAi 株系,对转基因植株根尖进行大样本(n 100)显微 观察。统计结果显示:转基因植株根尖径向生长与对照相比具有极显著差异,外源施用GA3 与赤霉素合成抑制剂 PAC 可以逆转这种差异,表明赤霉素对根尖径向生长具有负调控作用;此外,利用TobRB7 启动子在根尖分生组织 特异上调和下调内源赤霉素水平可以得到与组成型调控转基因植株相似的表型,表明赤霉素对根尖径向生长的负 调控作用主要是通过对根尖分生组织生长发育的调控实现的。

     

    Abstract: We conducted our study on tobacco plants for altered rates of biosynthesis and catabolism of GA constitutively or in specific tissues by transgenic approach and RNAi gene silencing technology. In the present study, PtGA20ox and PtGA2ox1 were over-expressed under the control of 35S promoter or root meristem-specific promoter (TobRB7) respectively and NtGA20ox was silenced. Root tips of transgenic plants and NtGA20ox RNAi lines were microscopic observation (n 100). It showed that transgenic plants compared with control had a very significant difference. This difference can be reversed by the exogenous application of GA3 and gibberellin synthesis inhibitor PAC. Moreover, similar phenotypes were obtained for root tip meristem-specific up-and down-regulation and constitutive regulation of endogenous gibberellin levels. The results provide evidence that GA negative regulates the root tip diameter through effects on the meristem.

     

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