Distribution of Ca2+ in the stigma and style of Robinia pseudoacacia during pollen germination and pollen tube elongation.

SUN Peng; DAI Li; XU Zhao-he; SUN Fei; YUAN Cun-quan; HU Rui-yang; SUN Yu-han; LI Yun

doi:10.13332/j.1000-1522.20140394

Journal of Beijing Forestry University > 2015 > 37(11): 59-68. > DOI: 10.13332/j.1000-1522.20140394

SUN Peng, DAI Li, XU Zhao-he, SUN Fei, YUAN Cun-quan, HU Rui-yang, SUN Yu-han, LI Yun. Distribution of Ca2+ in the stigma and style of Robinia pseudoacacia during pollen germination and pollen tube elongation.[J]. Journal of Beijing Forestry University, 2015, 37(11): 59-68. DOI: 10.13332/j.1000-1522.20140394

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Distribution of Ca2+ in the stigma and style of Robinia pseudoacacia during pollen germination and pollen tube elongation.

1.
1 National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Biotechnology,Beijing Forestry University, Beijing, 100083, P. R. China;
2.
2 Non-timber Forest Research and Development Center of Chinese Academy of Forestry, Zhengzhou, Henan, 450003, P. R. China;
3.
3 College of Forestry, Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing, 100083, P. R. China;

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Received Date: October 27, 2014
Published Date: November 29, 2015

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Abstract

Abstract

We used antimonate precipitation in combination with transmission electron microscope (TEM) in order to investigate the distribution of Ca2+ in stigma and style of black locust (Robinia pseudoacacia) and its effect on pollen germination and pollen tube growth. Experimental materials were emasculated flowers of black locust with artificial pollination and non-pollination as control. Our findings were as follows. Before germination, a lot of Ca2+ was observed on and near the germinal pore. Pollination could increase the amount of Ca2+ in exudate vesicles and extracellular matrix on stigma. With the finishing of most pollen germination, the amount of Ca2+ in exudate vesicles started to decline; however, no obvious decrease occurred in extracellular matrix, indicating that the amount of Ca2+ in vesicles might promote pollen germination. A hollow stylar canal surrounded by a layer of canal cells was inside the style of black locust. Whether pollinated or not, lots of Ca2+ accumulated in the vacuoles and on cell walls of canal cells. Those Ca2+ may promote the pollen tube growth in vivo. Gradient distribution of Ca2+ in the style of black locust could not be observed. The results of this study are beneficial to revealing the distribution of Ca2+ in the stigma and style of black locust as well as other leguminous plants on pollen germination and tube elongation.
- Robinia pseudoacacia,
- stigma,
- style,
- distribution of Ca2+,
- pollen germination,
- pollen tube growth

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References (37)

References

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Distribution of Ca2+ in the stigma and style of Robinia pseudoacacia during pollen germination and pollen tube elongation.

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