Effects of phosphorus stress on photosynthesis and nitrogen assimilation of Fraxinus mandshurica seedlings

YANG Bo-wen; SUN Hai-long; WU Chu

doi:10.13332/j.1000-1522.20140417

Journal of Beijing Forestry University > 2015 > 37(8): 18-23. > DOI: 10.13332/j.1000-1522.20140417

YANG Bo-wen, SUN Hai-long, WU Chu. Effects of phosphorus stress on photosynthesis and nitrogen assimilation of Fraxinus mandshurica seedlings[J]. Journal of Beijing Forestry University, 2015, 37(8): 18-23. DOI: 10.13332/j.1000-1522.20140417

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Effects of phosphorus stress on photosynthesis and nitrogen assimilation of Fraxinus mandshurica seedlings

1.
1 College of landscape, Northeast Forestry University, Harbin, 150040, P. R. China;
2.
2 School of Forestry, Northeast Forestry University, Harbin, 150040, P. R. China;
3.
3 Faculty of Horticulture & Gardening, Yangtze University, Jingzhou, 434025, P. R. China

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Received Date: November 13, 2014
Revised Date: November 13, 2014
Published Date: August 30, 2015

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Abstract

Abstract

The aim of this study was to investigate the effects of phosphorus (P) deficiency on the photosynthetic rate and nitrogen assimilation of Fraxinus mandshurica seedlings. One-year-old F. mandshurica seedlings were grown in sand medium and supplied with P at four different concentrations (0.125, 0.25, 0.5 and 1mmol/L). The photosynthetic rates of F. mandshurica seedlings decreased as the P concentration decreased. The maximum net photosynthetic rate at the lowest P concentration (0.125mmol/L) was 61% of that at the highest P concentration (1mmol/L). The light compensation points were higher at lower P concentrations (0.125, 0.25 and 0.5mmol/L) than at the highest P concentration (1mmol/L). At all four P concentrations, the concentrations of total nitrogen, nitrate, and ammonium were higher in shoots than in roots. As the P concentration decreased, the nitrate concentrations in leaves and roots also decreased, suggesting that nitrate uptake was limited under low-P conditions. The ammonium concentrations in roots were higher at low P concentrations (0.125, 0.25 and 0.5mmol/L) than at the highest P concentration (1mmol/L), suggesting that ammonium uptake by seedlings increased under low-P conditions. Nevertheless, the total nitrogen concentrations in leaves decreased as the P concentration decreased. Under lower P concentrations (0.125 and 0.25mmol/L), nitrate reductase activity was lower in shoots than in roots, while the opposite trend was observed under higher P concentrations (0.5 and 1mmol/L). As the P concentration decreased, the activities of nitrate reductase in leaves and roots and aspartate aminotransferase in leaves decreased, while there were smaller changes in glutamine synthetase activity. These results suggeste that nitrogen uptake, the activities of nitrogen assimilation enzymes, and nitrogen allocation to leaves of F. mandshurica seedlings decrease under low-P conditions, resulting in a decreased photosynthetic rate.
- phosphorus,
- Fraxinus mandshurica,
- photosynthesis,
- nitrogen assimilation

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References (42)

References

[1]	GRACIANO C, TAMBUSSI E, CASTÁN E, et al. Dry mass partitioning and nitrogen uptake by Eucalyptus grandis plants in response to localized or mixed application of phosphorus[J]. Plant and Soil, 2009, 319(1-2): 175-184.
[1]	YUAN X Y,GUO P Y,HUANG J,et al. Effect of glyphosate on photosynthesis and chlorophyll fluorescence of leaves of glyphosate-resistant soybean( Glycine max ( L.) Merr.)seedlings under phosphorus deficiency stress[J]. Journal of Plant Nutrition and Fertilizer, 2014, 20(1): 221-228.
[2]	OLIVERA M, TEJERA N, IRIBARNE C, et al. Growth, nitrogen fixation and ammonium assimilation in common bean ( Phaseolus vulgaris ): effect of phosphorus[J]. Physiologia Plantarum, 2004, 121(3): 498-505.
[2]	GUO S L,YAN X F,BAI B,et al. Responses of larch seedling's photosynthetic characteristics to nitrogen and phosphorus deficiency[J]. Chinese Journal of Applied Ecology, 2005, 16(4): 589-594.
[3]	FAN W G,WANG L X. Photosynthetic response to different phosphorus levels on young Newhall navel orange trees[J]. Journal of Fruit Science, 2012, 29(2): 166-170.
[3]	WARREN C R, ADAMS M A. Phosphorus affects growth and partitioning of nitrogen to Rubisco in Pinus pinaster [J]. Tree Physiology, 2002, 22(1): 11-19.
[4]	WU C,WANG Z Q,SUN H L,et al. Effects of different concentrations of nitrogen and phosphorus on chlorophyll biosynthesis, chlorophyll a fluorescence, and photosynthetic rate in Larix olgensis seedlings[J]. Scientia Silvae Sinicae, 2005, 41(4): 31-36.
[4]	BOWN H E, WATT M S, CLINTON T W, et al. Partititioning concurrent influences of nitrogen and phosphorus supply on photosynthetic model parameters of Pinus radiata [J]. Tree Physiology, 2007, 27(3): 335-344.
[5]	BLOOMFIELD K J, FARQUHAR G D, LLOYD J. Photosynthesis-nitrogen relationships in tropical forest tree species as affected by soil phosphorus availability: a controlled environment study[J]. Functional Plant Biology, 2014, 41(8): 820-832.
[5]	WU C,FAN Z Q,WANG Z Q. Effect of phosphorus stress on chlorophyll biosynthesis, photosynthesis and biomass partitioning pattern of Fraxinus mandchurica seedlings[J]. Chinese Journal of Applied Ecology, 2004, 15(6): 935-940.
[6]	REICH P, OLEKSYN J, WRIGHT I. Leaf phosphorus influences the photosynthesis-nitrogen relation: a cross-biome analysis of 314 species[J]. Oecologia, 2009, 160(2): 207-212.
[6]	WU C,FAN Z Q,WANG Z Q. Influences of nitrogen and phosphorus supply on their absorption and growth of Fraxinus mandshurica seedlings[J]. Scientia Silvae Sinicae, 2005, 41(5): 196-200.
[7]	原向阳,郭平毅,黄洁,等. 缺磷胁迫下草甘膦对抗草甘膦大豆幼苗光合作用和叶绿素荧光参数的影响[J]. 植物营养与肥料学报, 2014, 20(1): 221-228.
[7]	LI H S. Principles and techniques of plant physiological biochemical experiment[M]. Beijing: Higher Education Press, 2000: 56-98.
[8]	WARREN C R. How does P affect photosynthesis and metabolite profiles of Eucalyptus globulus [J]. Tree Physiology, 2011, 31(7): 727-739.
[8]	ZHANG Y D,FAN Z Q,WANG Q C,et al. Effect of different nitrogen forms on growth of Fraxinus mandshurica seedlings[J]. Chinese Journal of Applied Ecology, 2000, 11(10): 665-667.
[9]	郭盛磊,阎秀峰,白冰,等. 落叶松幼苗光合特性对氮和磷缺乏的响应[J]. 应用生态学报, 2005, 16(4): 589-594.
[9]	ZHANG Y,CUI X Y. Nitrogen absorption and assimilation characteristics of Pinus koraiensis seedlings in different N H 4 + /N O 3 - ratios[J]. Journal of Beijing Forestry University, 2011, 33(5):61-64.
[10]	GUO Y P,CHEN P Z,ZHANG L C,et al. Effects of different phosphorus nutrition levels on photosynthesis in satsuma mandarin ( Citrus unshiu Marc. ) leaves[J]. Plant Nutrition and Fertilizer Science, 2002, 8(2): 186-191.
[10]	樊卫国,王立新. 纽荷尔脐橙幼树对不同供磷水平的光合响应[J]. 果树学报, 2012, 29(2): 166-170.
[11]	RUFTY T W, MACKOWN C T, ISRAEL D W. Phosphorus stress effects on assimilation of nitrate[J]. Plant Physiology, 1990, 94(1): 328-333.
[12]	GNIAZDOWSKA A, RYCHTER A. Nitrate uptake by bean ( Phaseolus vulgaris L.) roots under phosphate deficiency[J]. Plant and Soil, 2000, 226(1): 79-85.
[13]	BLOOMFIELD K J, FARQUHAR G D, LLOYD J. Photosynthesis-nitrogen relationships in tropical forest tree species as affected by soil phosphorus availability: a controlled environment study[J]. Functional Plant Biology, 2014, 41(8): 820-832.
[14]	吴楚,王政权,孙海龙,等. 氮磷供给对长白落叶松叶绿素合成、叶绿素荧光和光合速率的影响[J]. 林业科学, 2005, 41(4): 31-36.
[15]	BOYCE R L, LARSON J R, SANFORD R L. Phosphorus and nitrogen limitations to photosynthesis in Rocky Mountain bristlecone pine ( Pinus aristata ) in Colorado[J]. Tree Physiology, 2006, 26(11): 1477-1486.
[16]	WALKER A P, BECKERMAN A P, GU L, et al. The relationship of leaf photosynthetic traits-V cmax and J max - to leaf nitrogen, leaf phosphorus, and specific leaf area: a meta-analysis and modeling study[J]. Ecology and Evolution, 2014, 4(16): 3218-3235.
[17]	TISCHNER R. Nitrate uptake and reduction in higher and lower plants[J]. Plant, Cell and Environment. 2000, 23(10): 1005-1024.
[18]	GRADOWSKI T, THOMAS S C. Phosphorus limitation of sugar maple growth in central Ontario[J]. Forest Ecology and Management, 2006, 226: 104-109.
[19]	RICHARDS A E, FORRESTER D I, BAUHUS J, et al. The influence of mixed tree plantations on the nutrition of individual species: a review[J]. Tree Physiology, 2010, 30 (9): 1192-1208.
[20]	TISSUE D T, LEWIS J D. Photosynthetic responses of cottonwood seedlings grown in glacial through future atmospheric [CO 2 ] vary with phosphorus supply[J]. Tree Physiology, 2010, 30(11): 1361-1372.
[21]	吴楚,范志强,王政权. 磷胁迫对水曲柳幼苗叶绿素合成、光合作用和生物量分配格局的影响[J]. 应用生态学报, 2004, 15(6): 935-940.
[22]	吴楚,范志强,王政权. 氮磷供应状态对水曲柳幼苗氮磷吸收与生长的影响[J]. 林业科学, 2005, 41(5): 196-200.
[23]	MR B, ESCOBAR-GUTIÉRREZ A, BURNS A, et al. Nitrogen-limited growth of lettuce is associated with lower stomatal conductance[J]. New Phytologist, 2001, 152: 97-106.
[24]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000: 56-98.
[25]	KNOEPP J D, TURNER D P, TINGEY D T. Effects of ammonium and nitrate on nutrient uptake and activity of nitrogen assimilating enzymes in western hemlock[J].Forest Ecology and Management, 1993, 59: 179-191.
[26]	HUSTED S, HEBBERN C A, MATTSSON M, et al. A critical experimental evaluation of methods for determination of N H 4 + in plant tissue, xylem sap and apoplastic fluid[J]. Physiologia Plantarum, 2000, 109(2): 167-179.
[27]	GNIAZDOWSKA A, MIKULSKA M, RYCHTER A M. Growth, nitrate uptake and respiration rate in bean roots under phosphate deficiency[J]. Biologia Plantarum, 1998, 41(2): 217-226.
[28]	张彦东,范志强,王庆成,等. 不同形态N素对水曲柳幼苗生长的影响[J]. 应用生态学报. 2000, 11(10): 665-667.
[29]	YUAN H, LIU D. Signaling components involved in plant responses to phosphate starvation[J]. Journal of Integrative Plant Biology, 2008, 50(7): 849-859.
[30]	ROTHSTEIN D E, CREGG B M. Effects of nitrogen form on nutrient uptake and physiology of Fraser fir( Abies fraseri )[J]. Forest Ecology and Management, 2005, 219: 69-80.
[31]	张韫,崔晓阳. 不同N H 4 + /N O 3 - 配比作用下红松幼苗的氮吸收和同化特征[J]. 北京林业大学学报, 2011, 33(5): 61-64.
[32]	郭延平,陈屏昭,张良诚,等. 不同供磷水平对温州蜜柑叶片光合作用的影响[J]. 植物营养与肥料学报, 2002, 8(2): 186-191.

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