Analysis of the expression patterns of gene induced by abiotic stress and signal in birch.

ZHOU Shan; LI Si-da; DONG Heng; ZHAN Ya-guang; ZENG Fan-suo.

doi:10.13332/j.1000-1522.20140485

Journal of Beijing Forestry University > 2015 > 37(11): 69-75. > DOI: 10.13332/j.1000-1522.20140485

ZHOU Shan, LI Si-da, DONG Heng, ZHAN Ya-guang, ZENG Fan-suo.. Analysis of the expression patterns of gene induced by abiotic stress and signal in birch.[J]. Journal of Beijing Forestry University, 2015, 37(11): 69-75. DOI: 10.13332/j.1000-1522.20140485

Citation:

PDF (1319 KB)

Analysis of the expression patterns of gene induced by abiotic stress and signal in birch.

College of Life science, Northeast Forestry University, Harbin, Heilongjiang, 150040, P.R. China;

More Information

Received Date: December 04, 2014
Published Date: November 29, 2015

Graphical Abstract

Abstract

Abstract

Heme oxygenase (HO) is the rate-limiting enzyme in the process of heme catabolism which is ubiquitous, sensitive, and highly active . This paper reveals the BpHO expression pattern in signal induction and regulation of secondary metabolism, in an attempt to lay the foundation for the study of gene function in metabolic regulation of birch. In our previous research, the HO gene has been cloned from birch (Betula platyphylla), named BpHO. The molecular structure of BpHO was analyzed by bioinformatics software. Abiotic stress by cadmium (Cd), salt (NaCl) and low temperature (4 ℃), and signal induced by sodium nitroprusside (SNP) and salicylic acid (SA) were used in the study. The expression patterns of BpHO induced by abiotic and signal induction were analyzed. Bioinformatics analysis showed that BpHO is 855 bp, and contains the complete ORF, encoding 284 amino acids (Genebank ID:KJ197335). BpHO is an unstable hydrophobic protein, which does not possess signal peptide and the transmembrane ability either. The alpha helix, extension chain and random coil are distributed throughout the protein. Molecular evolution analysis results showed that the genetic distance of BpHO gene between birch and grape species is relatively close. The gene heredity distance is farther with soybean, Kidney bean and pea, explaining the far genetic relationship with them. Abiotic stress results indicated that BpHO gene expression levels varied with abiotic stress time with fluctuations, but after 6 h treatment BpHO gene for four abiotic stress treatments were up-regulated, indicating BpHO genes are responsive to abiotic stresses. The results suggest that the signal induced by exogenous NO regulates transcription expression of BpHO and the synthesis of heme oxygenase. Salicylic acid is also involved in expression of BpHO. Salt, low temperature, heavy metal, and cadmium stress can promote the expression of BpHO, while the response patterns differ.
- Betula platyphylla,
- BpHO,
- bioinformatics analysis,
- abiotic stress

FullText(HTML)

References (30)

References

[1]	CUI W, LI L, GAO Z, et al. Haem oxygenase-1 is involved in salicylic acid-induced alleviation of oxidative stress due to cadmium stress in Medicago sativa[J]. Journal of Experimental Botany, 2012, 63(15): 5521-5534.
[1]	ZHOU S, SUN F K, JIANG T, et al. Bioinformatics and expression pattern analysis of BpNOS gene in Betula platyphylla[J]. Chinese Traditional and Herbal Drugs,2015,46(8):1203-1208.
[2]	CAO Z, GENG B, XU S, et al. BnHO1, a haem oxygenase-1 gene from Brassica napus, is required for salinity and osmotic stress-induced lateral root formation[J]. Journal of Experimental Botany, 2011, 62(13): 4675-4689.
[2]	YIN J, ZHAN Y G, XIAO J L. Synthesis and regulation of triterpene from Betula platyphylla Suk[J]. Plant Physiology Communications, 2009,45 (5): 520-526.
[3]	ZHENG C F, JIANG D, DAI Y B, et al. Effects nitroprusside,a nitric oxide donor, on carbon and nitrogen metabolism and the activity of the antioxidation system in wheat seedings under salt stress[J]. Acta Ecologica Sinica, 2010, 30(5): 1174-1183.
[3]	WU M, HUANG J, XU S, et al. Haem oxygenase delays programmed cell death in wheat aleurone layers by modulation of hydrogen peroxide metabolism[J]. Journal of Experimental Botany, 2011, 62(1): 235-248.
[4]	BAUDOUIN E, FRENDO P, LE GLEUHER M, et al. A Medicago sativa haem oxygenase gene is preferentially expressed in root nodules[J]. Journal of Experimental Botany, 2004, 55(394): 43-47.
[4]	SUN D Z, HE S P, PENG J, et al. Effects of salicylic acid and sodium nitropprusside on tomato seedling growth and physiological characteristics under NaCl stress[J]. Acta Botanica Boreali-Occidentalia Sinica,2013, 33(3): 541-546.
[5]	LIANG N S, ZHOU S, LI L L, et al. Cloning and analysis of bHLH transcription factor genes and promoters in Populus × xiaohei T. S. Hwang et Liang[J]. Nonwood Forest Research,2013, 31(4):58-66.
[5]	SHEKHAWAT G S, VERMA K. Haem oxygenase (HO): an overlooked enzyme of plant metabolism and defence[J]. Journal of Experimental Botany, 2010, 61(9): 2255-2270.
[6]	ZHANG Q Y, LIU F C, DUAN K, et al. Effects of salicylic acid on the expression of FaNBS20 gene responsive to Colletotrichum gloeosporioides infection in Fragaria×ananassa[J]. Acta Horticulturae Sinica, 2014, 41(1): 53-62
[6]	XUAN W, ZHU F Y, XU S, et al. The heme oxygenase/carbon monoxide system is involved in the auxin-induced cucumber adventitious rooting process[J]. Plant Physiology, 2008, 148(2): 881-893.
[7]	周姗,孙丰坤,姜涛,等. 白桦BpNOS基因的生物信息及表达模式分析[J]. 中草药,2015,46(8):1203-1208.
[8]	CUI Y, XU G, WANG M, et al. Expression of OsMSR3 in Arabidopsis enhances tolerance to cadmium stress[J]. Plant Cell Tissue Organ Culture, 2013, 113(2):331-340.
[9]	MA L J, LI X M, BU N, et al. An alginate-derived oligosaccharide enhanced wheat tolerance to cadmium stress[J]. Plant Growth Regulation, 2010, 62(1):71-76.
[10]	KHURANA A, KUMR R, BABBAR S B. Nitric oxide is involved in salicylic acid-induced flowering of Lemna aequinoctialis Welw[J]. Acta Physiologiae Plantarum, 2014, 36(10):2827-2833.
[11]	FENG H Q, GUAN D D, BAI J Y, et al. Extracellular ATP alleviates the salicylic acid-induced inhibition of cell viability and respiration through a Ca2+-dependent mechanism[J]. Biologia Plantarum, 2015, 59(1):193-197.
[12]	尹静, 詹亚光, 肖佳雷. 白桦三萜的合成和调控[J]. 植物生理学通讯, 2009,45 (5): 520-526.
[13]	XU Y, SUN X, JIN J, et al. Protective effect of nitric oxide on light-induced oxidative damage in leaves of tall fescue[J]. Journal of Plant Physiology, 2010, 167(7): 512-518.
[14]	郑春芳, 姜东, 戴廷波, 等. 外源一氧化氮供体硝普钠浸种对盐胁迫下小麦幼苗碳氮代谢及抗氧化系统的影响[J]. 生态学报, 2010, 30(5): 1174-1183.
[15]	孙德智, 何淑平, 彭靖, 等. 水杨酸和硝普钠对NaCl胁迫下番茄幼苗生长及生理特性的影响[J].西北植物学报,2013, 33(3): 541-546.
[16]	JOHN M W. The proteomics protocols handbook[M]. Totowa: Humana Press, 2005: 571-607.
[17]	THOMAS N P, SOREN B, GUNNAR H. SignalP 4.0: discriminating signal peptides from transmembrane regions[J]. Nature Methods, 2011,8(10): 785-786.
[18]	HOFMANN K, STOFFEL W. TMbase-A database of membrane spanning proteins segments[M]. Biol Chem Hoppe-Seyler, 1993, 374: 166.
[19]	SEN T Z, JERNIGAN R L, GARNIER J, et al. GOR V server for protein secondary structure prediction[J]. Bioinformatics, 2005, 21:2787-2788.
[20]	TAMURA K, DUDLEY J, NEI M. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0[J]. Molecular Biology and Evolution, 2007,24(8): 1596-1599.
[21]	梁楠松, 周姗, 李蕾蕾,等. 小黑杨bHLH转录因子基因和启动子的克隆与分析[J]. 经济林研究, 2013, 31(4):58-66.
[22]	ZHAO M G, TIAN Q Y, ZHANG W H. Nitric oxide synthase-dependent nitric oxide production is associated with salt tolerance in Arabidopsis[J]. Plant Physiology, 2007, 144(1): 206-217.
[23]	BOSE J, XIE Y, SHEN W, et al. Haem oxygenase modifies salinity tolerance in Arabidopsis by controlling K+ retention via regulation of the plasma membrane H+-ATPase and by altering SOS1 transcript levels in roots[J]. Journal of Experimental Botany, 2013, 64(2): 471-481.
[24]	张庆雨, 刘芳春, 段可, 等. 水杨酸对草莓炭疽病响应基因 FaNBS20 表达的影响[J]. 园艺学报, 2014, 41(1): 53-62.

[1]	Chen Beibei, Jiang Jun, Lu Yuanchang, Liu Xianzhao, Jia Hongyan, Ming Angang, Zhang Xianqiang. Effects of thinning intensity on the growth of interplanting broadleaved trees under Pinus massoniana plantation[J]. Journal of Beijing Forestry University, 2021, 43(1): 58-65. DOI: 10.12171/j.1000-1522.20200086
[2]	Zhang Xiaoyan, Li Yufei, Liu Guihua, Xu Zaoshi, Deng Bo. Effects of nitrogen application on growth and triterpenoids accumulation of 1-year-old Cyclocarya paliurus[J]. Journal of Beijing Forestry University, 2020, 42(4): 60-68. DOI: 10.12171/j.1000-1522.20190294
[3]	Chang Xiaochao, Liu Yong, Li Jinyu, Li Shian, Sun Minghui, Wan Fangfang, Zhang Jin, Song Xiehai. Effects of different nitrogen forms and ratios on growth of male Populus tomentosa seedlings[J]. Journal of Beijing Forestry University, 2018, 40(9): 63-71. DOI: 10.13332/j.1000-1522.20180178
[4]	Wang Junxiu, Zhou Yangyan, Han Xiao, An Yi, Guo Huihong, Xia Xinli, Yin Weilun, Liu Chao. Overexpression of Populus SBPase gene promoting photosynthesis and vegetative growth in Arabidopsis thaliana[J]. Journal of Beijing Forestry University, 2018, 40(3): 26-33. DOI: 10.13332/j.1000-1522.20170436
[5]	Yan Ya-ping, Liu Yong, He Guo-xin, Xue Dun-meng, Li Cheng. Coupling effects of water and fertilizer on seedling growth and nutrient status of Catalpa bungei[J]. Journal of Beijing Forestry University, 2018, 40(2): 58-67. DOI: 10.13332/j.1000-1522.20170251
[6]	ZHAO Yang, QIAO Jie, WANG Bao-ping, FENG Yan-zhi, ZHOU Hai-jiang, CUI Ling-jun, WANG Wei-wei, YANG Dai-gui. Comprehensive selection of growth and stem form of superior paulownia clones in the hilly region of southern China[J]. Journal of Beijing Forestry University, 2017, 39(9): 32-40. DOI: 10.13332/j.1000-1522.20160372
[7]	REN Shi-qi, CHEN Jian-bo, DENG Zi-yu, GUO Dong-qiang, LIU Yuan, HUANG Ming-jun, MENG Jiang-long, XIAO Wen-fa, XIANG Dong-yun. Effects of pruning on growth dynamic and veneer quality of Eucalyptus urophylla×E. grandis[J]. Journal of Beijing Forestry University, 2015, 37(3): 126-132. DOI: 10.13332/j.1000-1522.20140228
[8]	YANG Teng, DUAN Jie, MA L&uuml, -yi, JIA Li-ming, PENG Zuo-deng, CHEN Chuang, CHEN Jing. Effects of N application rates on growth, nutrient accumulation and translocation of Xanthoceras sorbifolia[J]. Journal of Beijing Forestry University, 2014, 36(3): 57-62. DOI: 10.13332/j.cnki.jbfu.2014.03.008
[9]	YANG Li-xue, WANG Hai-nan, FAN Jing. Effects of donor tree ages and plant growth regulators on the softwood cutting propagation of Hippophae rhamnoides[J]. Journal of Beijing Forestry University, 2011, 33(6): 107-111.
[10]	HAN Lie-bao, WANG Chang-jun, SU De-rong, JIANG Yan-ling, XU Jun, ZHOU Jun. Accumulation and comparison of greenbelt nutrients under different water irrigating ways[J]. Journal of Beijing Forestry University, 2005, 27(6): 62-66.

Cited By

Get Citation

PDF

XML

Article views (1840) PDF downloads (33)

Turn off MathJax

Article Contents

Abstract

References

Analysis of the expression patterns of gene induced by abiotic stress and signal in birch.

Abstract

References

Related Articles

Catalog

Analysis of the expression patterns of gene induced by abiotic stress and signal in birch.

Abstract

References

Related Articles

Catalog

Export File

Citation

Format

Content