• Scopus
  • Chinese Science Citation Database (CSCD)
  • A Guide to the Core Journal of China
  • CSTPCD
  • F5000 Frontrunner
  • RCCSE
Advanced search
Yang Shu-juan, You Yan-zhi, Zhang Wei-wei, Wang Kun, Jiang Jian-xin. NaOH-ethanol pretreatment increasing preparation efficiency of xylo-oligosaccharide from sugarcane bagasse with enzymatic hydrolysis[J]. Journal of Beijing Forestry University, 2018, 40(2): 114-120. DOI: 10.13332/j.1000-1522.20170366
Citation: Yang Shu-juan, You Yan-zhi, Zhang Wei-wei, Wang Kun, Jiang Jian-xin. NaOH-ethanol pretreatment increasing preparation efficiency of xylo-oligosaccharide from sugarcane bagasse with enzymatic hydrolysis[J]. Journal of Beijing Forestry University, 2018, 40(2): 114-120. DOI: 10.13332/j.1000-1522.20170366

NaOH-ethanol pretreatment increasing preparation efficiency of xylo-oligosaccharide from sugarcane bagasse with enzymatic hydrolysis

More Information
  • Received Date: October 11, 2017
  • Revised Date: November 29, 2017
  • Published Date: January 31, 2018
  • ObjectiveThe condition of preparation of xylo-oligosaccharide (XOS) from sugarcane bagasse with enzymatic hydrolysis was investigated. In this study, the preparation efficiency of xylo-oligosaccharide was improved by NaOH-ethanol pretreatment.
    MethodFirstly, the chemical composition of the sugarcane bagasse before and after pretreatment was characterized, and the effect of pretreatment on the components of the raw material was determined. Secondly, the wettability and physical structure of substrates were characterized by contact angle analysis and X-ray diffraction (XRD). Finally, in order to compare the effects of different pretreatments on the mass concentration of XOS with enzymatic hydrolysis, the enzymatic hydrolysate was harvested and analyzed by high performance liquid chromatography (HPLC).
    ResultCharacterization results confirmed that optimal pretreatment condition was 10 g/L NaOH-ethanol (50% v/v ethanol) pretreatment. Under this pretreatment condition, a large amount of lignin was removed and the removal rate of lignin could reach 78.10%. Moreover, the pretreatment could effectively improve the hydrophilicity of raw materials, decreased the contact angle from 61.5° to 55.4°, and also increased the crystallinity of lignocellulose from 28.6% to 32.3%.The results showed that the highest XOS mass concentration was 1.85 g/L after 10 g/L NaOH-ethanol pretreatment, which was 122.89% higher than that of untreated sugarcane bagasse (0.83 g/L).
    ConclusionIn the process of XOS preparation from sugarcane bagasse, enzymatic hydrolysis was an efficient way to achieve the conversion from hemicellulose to XOS. NaOH-ethanol pretreatment can effectively increase the efficiency of enzymatic hydrolysis and promote the preparation of XOS.
  • [1]
    Chen M H, Bowman M J, Dien B S, et al. Autohydrolysis of Miscanthus×giganteus for the production of xylooligosaccharides (XOS): kinetics, characterization and recovery[J]. Bioresource Technology, 2014, 155(2): 359-365.
    [2]
    Shin J H, Choi J H, Lee O S, et al. Thermostable xylanase from Streptomyces thermocyaneoviolaceus for optimal production of xylooligosaccharides[J]. Biotechnology and Bioprocess Engineering, 2009, 14(4): 391-399. doi: 10.1007/s12257-008-0220-3
    [3]
    石波, 李里特.低聚木糖的制备与分离[J].食品工业科技, 2004, 25(7): 113-114. doi: 10.3969/j.issn.1002-0306.2004.07.045

    Shi B, Li L T. Study on the preparation and isolation of xylooligosacchrides[J]. Science and Technology of Food Industry, 2004, 25(7): 113-114. doi: 10.3969/j.issn.1002-0306.2004.07.045
    [4]
    Vallejos M E, Zambon M D, Area M C, et al. Low liquid-solid ratio (LSR) hot water pretreatment of sugarcane bagasse[J]. Green Chemistry, 2012, 14(7): 1982-1989. doi: 10.1039/c2gc35397k
    [5]
    Parajó J C, Garrote G, Cruz J M, et al. Production of xylooligosaccharides by autohydrolysis of lignocellulosic materials[J]. Trends in Food Science & Technology, 2004, 15(3-4): 115-120. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=Doaj000004160294
    [6]
    Saha B C. Hemicellulose bioconversion[J]. Journal of Industrial Microbiology and Biotechnology, 2003, 30(5): 279-291. doi: 10.1007/s10295-003-0049-x
    [7]
    Pérez J, Muñoz-Dorado J, de la Rubia T, et al. Biodegradation and biological treatments of cellulose, hemicellulose and lignin: an overview[J]. International Microbiology, 2002, 5(2): 53-63. doi: 10.1007/s10123-002-0062-3
    [8]
    Xue J L, Zhao S, Liang R M, et al. A biotechnological process efficiently co-produces two high value-added products, glucose and xylooligosaccharides, from sugarcane bagasse[J]. Bioresource Technology, 2016, 204: 130-138. doi: 10.1016/j.biortech.2015.12.082
    [9]
    顾阳, 勇强, 余世袁.高温预处理对木聚糖酶水解制备低聚木糖的促进作用[J].林产化学与工业, 2006, 26(1): 6-10. doi: 10.3321/j.issn:0253-2417.2006.01.002

    Gu Y, Yong Q, Yu S Y. Promotional effect of high temperature pretreatment on enzymatic hydrolysis of xylan into xylooligosaccharide[J]. Chemistry and Industry of Forest Products, 2006, 26(1): 6-10. doi: 10.3321/j.issn:0253-2417.2006.01.002
    [10]
    Limayem A, Ricke S C. Lignocellulosic biomass for bioethanol production: current perspectives, potential issues and future prospects [J]. Progress in Energy and Combustion Science, 2012, 38(4): 449-467. doi: 10.1016/j.pecs.2012.03.002
    [11]
    秦书百川, 刘彦涛, 卜令习, 等.碱性亚硫酸盐耦合低压蒸汽预处理慈竹及其纤维素酶解转化[J].北京林业大学学报, 2016, 38(7): 98-104. doi: 10.13332/j.1000-1522.20150456

    Qin S B C, Liu Y T, Bu L X, et al. Alkaline sulfite and low-pressure steam coupled pretreatment on Neosinocalamus affinis and its enzymatic hydrolysis[J]. Journal of Beijing Forestry University, 2016, 38(7): 98-104. doi: 10.13332/j.1000-1522.20150456
    [12]
    Sluiter A, Hames B, Ruiz R, et al. Determination of structural carbohydrates and lignin in biomass[R]//NREL. Technical report NREL/TP-510-42618. Golden, Colorado, USA: National Renewable Energy Laboratory, 2012.
    [13]
    Yu H L, You Y Z, Lei F H, et al. Comparative study of alkaline hydrogen peroxide and organosolv pretreatments of sugarcane bagasse to improve the overall sugar yield[J]. Bioresource Technology, 2015, 187: 161-166. doi: 10.1016/j.biortech.2015.03.123
    [14]
    杨淑娟, 游艳芝, 卜令习, 等.废纸脱墨浆预处理及其酶解性能研究[J].生物质化学工程, 2016, 50(4): 37-41. doi: 10.3969/j.issn.1673-5854.2016.04.007

    Yang S J, You Y Z, Bu L X, et al. Pretreatment of waste paper pulp and enzymatic hydrolysis properties[J]. Biomass Chemical Engineering, 2016, 50(4): 37-41. doi: 10.3969/j.issn.1673-5854.2016.04.007
    [15]
    Aachary A A, Prapulla S G. Value addition to corncob: production and characterization of xylooligosaccharides from alkali pretreated lignin-saccharide complex using Aspergillus oryzae MTCC 5154[J]. Bioresource Technology, 2009, 100(2): 991-995. doi: 10.1016/j.biortech.2008.06.050
    [16]
    Morgan N K, Wallace A, Bedford M R, et al. Efficiency of xylanases from families 10 and 11 in production of xylo-oligosaccharides from wheat arabinoxylans[J]. Carbohydrate Polymers, 2017, 167: 290-296. doi: 10.1016/j.carbpol.2017.03.063
    [17]
    Azelee N I W, Jahim J M, Ismail A F, et al. High xylooligosaccharides (XOS) production from pretreated kenaf stem by enzyme mixture hydrolysis[J]. Industrial Crops and Products, 2016, 81: 11-19. doi: 10.1016/j.indcrop.2015.11.038
    [18]
    Si S L, Chen Y, Fan C F, et al. Lignin extraction distinctively enhances biomass enzymatic saccharification in hemicelluloses-rich Miscanthus species under various alkali and acid pretreatments[J]. Bioresource Technology, 2015, 183: 248-254. doi: 10.1016/j.biortech.2015.02.031
    [19]
    蒋建新, 杨中开, 朱莉伟, 等.竹纤维结构及其性能研究[J].北京林业大学学报, 2008, 30(1): 128-132. doi: 10.3321/j.issn:1000-1522.2008.01.023

    Jiang J X, Yang Z K, Zhu L W, et al. Structure and property of bamboo fiber[J]. Journal of Beijing Forestry University, 2008, 30(1): 128-132. doi: 10.3321/j.issn:1000-1522.2008.01.023
    [20]
    徐勇, 陈牧, 余世袁, 等.木聚糖酶水解制取低聚木糖的研究[J].林产化学与工业, 2002, 22(2): 57-60. doi: 10.3321/j.issn:0253-2417.2002.02.014

    Xu Y, Chen M, Yu S Y, et al. Study on enzymatic hydrolysis of xylan into xylo-oligosaccharide[J]. Chemistry and Industry of Forest Products, 2002, 22(2): 57-60. doi: 10.3321/j.issn:0253-2417.2002.02.014
    [21]
    蔡彦, 马玉龙, 谢丽, 等.不同预处理方法对麦草纤维素酶解效果的影响[J].可再生能源, 2010, 28(3): 72-74, 80. doi: 10.3969/j.issn.1671-5292.2010.03.016

    Cai Y, Ma Y L, Xie L, et al. Comparative study on pretreatment of wheat straw cellulose with compound method[J]. Renewable Energy Resources, 2010, 28(3): 72-74, 80. doi: 10.3969/j.issn.1671-5292.2010.03.016
    [22]
    叶利培, 房桂干, 沈葵忠, 等.木聚糖酶水解竹材废弃物制取低聚木糖工艺条件的优化[J].食品工业科技, 2013, 34(22): 141-144, 148. http://d.old.wanfangdata.com.cn/Periodical/spgykj201322033

    Ye L P, Fang G G, Shen K Z, et al. Optimization of xylo-oligosaccharides production by xylanase hydrolysis with bamboo wastes in processing[J]. Science and Technology of Food Industry, 2013, 34(22): 141-144, 148. http://d.old.wanfangdata.com.cn/Periodical/spgykj201322033
    [23]
    Yang S J, You Y Z, Li X L, et al. Effects of papermaking additives for effective hydrolysis of deinked waste paper[J]. Journal of Biobased Materials and Bioenergy, 2017, 11(2): 142-147. doi: 10.1166/jbmb.2017.1651
    [24]
    Reilly P J. Xylanases: structure and function[M]//Hollaender A, Rabson R, Rogers P, et al. Trends in the biology of fermentations for fuels and chemicals. Boston: Springer, 1981: 111-129.
    [25]
    Kumar S, Gupta R, Lee Y Y, et al. Cellulose pretreatment in subcritical water: effect of temperature on molecular structure and enzymatic reactivity[J]. Bioresource Technology, 2010, 101(4): 1337-1347. doi: 10.1016/j.biortech.2009.09.035
    [26]
    Yu H L, Tang Y, Xing Y, et al. Improvement of the enzymatic hydrolysis of furfural residues by pretreatment with combined green liquor and hydrogen peroxide[J]. Bioresource Technology, 2013, 147: 29-36. doi: 10.1016/j.biortech.2013.08.013
    [27]
    Zhao C, Ma Z Q, Shao Q J, et al. Enzymatic hydrolysis and physiochemical characterization of corn leaf after H-AFEX pretreatment[J]. Energy & Fuels, 2016, 30(2): 1154-1161. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=1e9e1185bfc0fcae841d56a9694a79d6
    [28]
    周玉恒, 陈海珊, 蔡爱华, 等.蒸汽爆破对甘蔗叶酶法制备低聚木糖的影响[J].食品与发酵工业, 2011, 37(2): 94-98. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=spyfx201102021

    Zhou Y H, Chen H S, Cai A H, et al. The influence of steam explosion on enzymatic preparation of xylooligosaccharides from sugarcane leaves[J]. Food and Fermentation Industries, 2011, 37(2): 94-98. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=spyfx201102021
    [29]
    田龙, 王云, 马晓建.小麦秸秆酶法制备低聚木糖及其抗氧化活性[J].食品科学, 2014, 35(20): 88-92. doi: 10.7506/spkx1002-6630-201420018

    Tian L, Wang Y, Ma X J. Preparation and antioxidant activity of xylooligosaccharides from wheat straw by enzymatic hydrolysis with xylanase[J]. Food Science, 2014, 35(20): 88-92. doi: 10.7506/spkx1002-6630-201420018
    [30]
    Jiang Z Q, Deng W, Zhu Y P, et al. The recombinant xylanase B of Thermotoga maritima is highly xylan specific and produces exclusively xylobiose from xylans, a unique character for industrial applications[J]. Journal of Molecular Catalysis B: Enzymatic, 2004, 27(4-6): 207-213. doi: 10.1016/j.molcatb.2003.11.012
  • Related Articles

    [1]Zhao Han, Wang Mingjie, He Linhan, Chen Yao, Gao Jianmin. Preparation and its electrochemical properties of enzymatically hydrolyzed wood-based hierarchical porous carbon[J]. Journal of Beijing Forestry University, 2024, 46(10): 136-143. DOI: 10.12171/j.1000-1522.20240026
    [2]Xie Chen, Zhang Yang. Effects of deep eutectic solvents pretreatment on performance of surface mineralized wood[J]. Journal of Beijing Forestry University, 2024, 46(7): 123-132. DOI: 10.12171/j.1000-1522.20240067
    [3]Li Kunyan, Du Mingjie, Zhong Yuan, Cheng Fangyun. Optimization of chromosome pretreatment method and its application in karyotype analysis of Paeonia × lemoinei ‘High Noon’[J]. Journal of Beijing Forestry University, 2021, 43(5): 118-126. DOI: 10.12171/j.1000-1522.20200301
    [4]Li Wanjin, Wang Ruoshui, Xiao Huijie, Wang Baitian, Zhang Kebin, Liu Qingqing, Guo Binghan. Effects of fresh seaweed bio-enzymatic organic fertilizer on the growth and soil physicochemical properties of Atraphaxis bracteata[J]. Journal of Beijing Forestry University, 2018, 40(7): 62-72. DOI: 10.13332/j.1000-1522.20170269
    [5]Liu Pengpeng, Zhou Sukun, Wang Chao, You Tingting, Xu Feng. Preparation and properties of mesoporous activated carbons from NaOH-pretreated corncob residues[J]. Journal of Beijing Forestry University, 2018, 40(3): 128-134. DOI: 10.13332/j.1000-1522.20170431
    [6]SUN Ya-jie, MA Yan-li, DAI Jun-xiu, REN Shi-xue, FANG Gui-zhen.. Preparation and characterization of enzymatic hydrolysis lignin-based hydrogel.[J]. Journal of Beijing Forestry University, 2016, 38(11): 97-103. DOI: 10.13332/j.1000-1522.20160095
    [7]QIN Shu-bai-chuan, LIU Yan-tao, BU Ling-xi, ZHAO Peng-xiang, JIANG Jian-xin. Alkaline sulfite and low-pressure steam coupled pretreatment on Neosinocalamus affinis and its enzymatic hydrolysis.[J]. Journal of Beijing Forestry University, 2016, 38(7): 98-104. DOI: 10.13332/j.1000-1522.20150456
    [8]LU Qi, MENG Yong-bin, XU Lei, ZHANG Zi-dong, LIU Ying, ZHANG Ying, ZU Yuan-gang.. Technology in preparation of ethanol from the enzymatic hydrolysate of Tara fiber residues.[J]. Journal of Beijing Forestry University, 2015, 37(12): 122-127. DOI: 10.13332/j.1000-1522.20150160
    [9]DENG Li-hong, XIAO Ling-ping, TANG Yong, SONG Xian-liang. Effects of pretreatment on enzymatic hydrolysis of cellulose for waste paper[J]. Journal of Beijing Forestry University, 2010, 32(3): 170-175.
    [10]WANG Kun, WANG Fang, JIANG Jian-xin, SUN Run-cang, LIU Sheng-ying, MA Ya-qi, SONG Xian-liang. Influence of steaming time during steam-explosion on the chemical composition,crystallinity and enzymatic hydrolysis of Lespedeza bicolor stalks[J]. Journal of Beijing Forestry University, 2009, 31(5): 121-125.
  • Cited by

    Periodical cited type(8)

    1. 袁然,梁映红,傅童成,胡生龙,汪盛,易自力,李蒙. 南荻水热液化耦合酶解联产多种纤维糖. 中国造纸. 2024(11): 71-80 .
    2. 张婷,孙德娇,王彩衣,黄鑫,陈少强,黎飞望,杨齐. 甘蔗渣制备低聚木糖的工艺优化. 广西科学. 2023(04): 727-734 .
    3. 童欣怡,李琦,陈文倩,赵林果. 杨木屑木聚糖碱法提取及其制备低聚木糖的工艺研究. 林业工程学报. 2020(01): 61-68 .
    4. 朱增科,平清伟,张健,盛雪茹,李娜,石海强. 乙醇-水抽提法分离毛竹枝桠材组分的研究. 中国造纸. 2020(08): 52-56 .
    5. 张威伟,张波,张乐平,蒋建新. 非酶法催化木质纤维原料转化制备低聚木糖研究进展. 林产化学与工业. 2020(06): 118-128 .
    6. 邓元元,胡超,陈思宇,朱文娟,王刚,兰时乐. 啤酒糟制备低聚木糖功能饲料添加剂酶解条件优化. 中国饲料. 2019(09): 54-62 .
    7. 陈冰炜,阚玉娜,袁诚,王新洲,黄曹兴,梅长彤,翟胜丞. 乙醇预处理对芦竹细胞壁的影响及荧光可视化分析. 林业工程学报. 2019(04): 59-65 .
    8. 刘洋,胡小文,姚艳丽. NaOH预处理对甘蔗渣成分和酶解效率的影响. 甘蔗糖业. 2019(06): 28-38 .

    Other cited types(4)

Catalog

    Article views (2037) PDF downloads (64) Cited by(12)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return