Effects of Cd-Zn combined stress on chemical defense of <i>Populus alba</i> ‘Berolinensis’

Wang Yueyue; Dong Xiaowen; Jiang Dun; Di Guiqiu; Wang Mo; Liu Xiaoxia; Meng Zhaojun; Yan Shanchun

doi:10.13332/j.1000-1522.20190008

Journal of Beijing Forestry University > 2019 > 41(6): 96-101. > DOI: 10.13332/j.1000-1522.20190008

Wang Yueyue, Dong Xiaowen, Jiang Dun, Di Guiqiu, Wang Mo, Liu Xiaoxia, Meng Zhaojun, Yan Shanchun. Effects of Cd-Zn combined stress on chemical defense of Populus alba ‘Berolinensis’[J]. Journal of Beijing Forestry University, 2019, 41(6): 96-101. DOI: 10.13332/j.1000-1522.20190008

Citation:

PDF (698 KB)

Effects of Cd-Zn combined stress on chemical defense of Populus alba ‘Berolinensis’

1.
School of Forestry, Northeast Forestry University, Harbin 150040, Heilongjiang, China
2.
Key Laboratory of Sustainable Forest Ecosystem Management of Ministry of Education, Northeast Forestry University, Harbin 150040, Heilongjiang, China

More Information

Received Date: January 11, 2019
Revised Date: March 04, 2019
Available Online: June 04, 2019
Published Date: May 31, 2019

Graphical Abstract

Abstract

Abstract

ObjectiveThis study aims to investigate the effects of Cd-Zn combined stress on chemical defense of Populus alba ‘Berolinensis ’ (PAB).
MethodThree concentrations of Cd (0.5, 1.5 and 2.5 mg/kg) and Zn (300, 500 and 700 mg/kg) were combined and applied in an orthogonal experimental design (3 × 3) into the soil with one-year-old PAB seedlings. The activities of defense proteins and the contents of secondary metabolites in the PAB leaves were analyzed at 30, 40 and 50 days after Cd-Zn applications, respectively.
ResultThe results showed that both the activities of defense proteins and the contents of secondary metabolites in the leaves of PAB seedlings decreased with the increase of Cd-Zn concentrations. Both parameters were significantly lower under the high Cd-Zn stresses than those under the medium-low Cd-Zn stresses (P < 0.05). The activities of PAL, PPO, TI, CI, and the contents of flavonoids and lignin in the PAB leaves under the high-medium Cd-Zn (combined) stresses were significantly lower than those of control (P < 0.05) for all three sampling dates (30, 40 and 50 days after the Cd-Zn application). Under the low Cd-Zn combined stresses, the flavonoid contents at 30 and 40 days after the Cd-Zn application and the PAL activity at 50 days after the Cd-Zn application were also significantly lower than those of control (P < 0.05).
ConclusionThe above results illustrate that Cd-Zn combined stress could inhibit the chemical defense levels of the PAB leaves, and such inhibitory effects vary, to certain degrees, with Cd-Zn concentrations and exposure durations. Further studies are need to determine how the Cd-Zn combined stresses affect PAB defoliators.
- combined heavy metal stress,
- Populus alba ‘Berolinensis’,
- Cd,
- Zn,
- chemical defense

FullText(HTML)

References (29)

References

[1]	李凤果, 陈明, 师艳丽, 等. 我国农用地土壤污染修复研究现状分析[J]. 现代化工, 2018, 38(12):4−9. Li F G, Chen M, Shi Y L, et al. Research progress of soil contamination restoration in agricultural land in China[J]. Modern Chemical Industry, 2018, 38(12): 4−9.
[2]	Kim H T, Kim J G. Uptake of cadmium, copper, lead, and zinc from sediments by an aquatic macrophyte and by terrestrial arthropods in a freshwater wetland ecosystem[J]. Archives of Environmental Contamination & Toxicology, 2016, 71(2): 1−12.
[3]	王锦文, 白秀, 陈锦峰, 等. 复合重金属Pb/Zn对香樟生理特征的影响[J]. 安徽农业科学, 2009, 37(21):10253−10254. doi: 10.3969/j.issn.0517-6611.2009.21.166 Wang J W, Bai X, Chen J F, et al. Effects of complex heavy metals Pb/Zn on the physiological characteristics of Paulowniu fortunel (seem) hems[J]. Journal of Anhui Agricultural Sciences, 2009, 37(21): 10253−10254. doi: 10.3969/j.issn.0517-6611.2009.21.166
[4]	Jia X, Zhao Y H, Liu T, et al. Leaf defense system of Robinia pseudoacacia L. seedlings exposed to 3 years of elevated atmospheric CO₂ and Cd-contaminated soils[J]. Science of The Total Environment, 2017, 605−606: 48−57.
[5]	杨期和, 赖万年, 杨和生, 等. 土壤Cd污染的生物修复[J]. 嘉应学院学报, 2014, 32(5):58−65. doi: 10.3969/j.issn.1006-642X.2014.05.012 Yang Q H, Lai W N, Yang H S, et al. Bioremediation of Cd contaminated soil[J]. Journal of Jiaying University, 2014, 32(5): 58−65. doi: 10.3969/j.issn.1006-642X.2014.05.012
[6]	杜锁军, 戴艳, 谢东俊, 等. 土壤Zn污染及其治理措施评述[J]. 污染防治技术, 2009, 22(3):87−90. Du S J, Dai Y, Xie D J, et al. The introduction of Zn pollution in soil and its control mesures[J]. Pollution Control Techonology, 2009, 22(3): 87−90.
[7]	吴志能, 谢苗苗, 王莹莹. 我国复合污染土壤修复研究进展[J]. 农业环境科学学报, 2016, 35(12):2250−2259. doi: 10.11654/jaes.2016-0863 Wu Z N, Xie M M, Wang Y Y. Remediation of soils with combined pollution in China: a review[J]. Journal of Agro-Environment Science, 2016, 35(12): 2250−2259. doi: 10.11654/jaes.2016-0863
[8]	刘艳丽, 吴凤霞, 徐莹, 等. 杨树修复重金属污染土壤的研究进展[J]. 林业科学, 2012, 48(9):139−144. Liu Y L, Wu F X, Xu Y, et al. Research progress in the remediation of heavy metal contaminated soils with Populus[J]. Scientia Silvae Sinicae, 2012, 48(9): 139−144.
[9]	齐洪涛, 白莉萍, 陆海波, 等. 堆肥污泥对不同杨树品种重金属富集变化的影响[J]. 东北林业大学学报, 2014, 42(11):22−27. doi: 10.3969/j.issn.1000-5382.2014.11.006 Qi H T, Bai L P, Lu H B, et al. Effects of fertilizer sludge on heavy metal concentration in different poplar varieties[J]. Journal of Northeast Forestry University, 2014, 42(11): 22−27. doi: 10.3969/j.issn.1000-5382.2014.11.006
[10]	国家环境保护局, 国家技术监督局. GB15618—1995 土壤环境质量标准[S]. 北京: 中国标准出版社, 1995. State Department of Environmental Conservation, State Bureau of Technical Supervision. GB15618 —1995 soil environmental quality standard[S]. Beijing: China Standards Press, 1995.
[11]	崔伟婵, 李霜雯, 严善春. 不同光照下外源茉莉酸类物质对兴安落叶松防御蛋白的影响[J]. 东北林业大学学报, 2016, 44(9):78−81. doi: 10.3969/j.issn.1000-5382.2016.09.017 Cui W C, Li S W, Yan S C. Effects of exogenous jasmonic acids on defense protein of Larix gmelinii under different illumination[J]. Journal of Northeast Forestry University, 2016, 44(9): 78−81. doi: 10.3969/j.issn.1000-5382.2016.09.017
[12]	姜虹, 严善春, 薛羿, 等. 落叶松林型对其针叶内几种防御蛋白活力和次生代谢物含量的影响[J]. 林业科学研究, 2018, 31(3):24−28. Jiang H, Yan S C, Xue Y, et al. Effects of forest type on activity of several defense proteins and contents of secondary metabolites in larch needles[J]. Forest Research, 2018, 31(3): 24−28.
[13]	王杰, 孟昭军, 王琪, 等. 外源茉莉酸诱导方式对长白落叶松针叶内防御蛋白活力的影响[J]. 东北林业大学学报, 2015, 43(5):84−89. doi: 10.3969/j.issn.1000-5382.2015.05.017 Wang J, Meng Z J, Wang Q, et al. Effects of exogenous jasmonic acid induction on defense protein activity in Larch needles[J]. Journal of Northeast Forestry University, 2015, 43(5): 84−89. doi: 10.3969/j.issn.1000-5382.2015.05.017
[14]	任琴, 胡永建, 李镇宇, 等. 受害马尾松木质素含量及其过氧化物酶活性[J]. 生态学报, 2007,27(11):4895−4899. doi: 10.3321/j.issn:1000-0933.2007.11.060 Ren Q, Hu Y J, Li Z Y, et al. Content variation of lignin and peroxidase activities from damaged Pinus massioniana[J]. Acta Ecologica Sinica, 2007,27(11): 4895−4899. doi: 10.3321/j.issn:1000-0933.2007.11.060
[15]	刘江, 周荣琪. 竹叶提取物总黄酮含量测定方法的改进[J]. 食品科技, 2005(7):76−79. doi: 10.3969/j.issn.1005-9989.2005.07.023 Liu J, Zhou R Q. Improved meansuration for general flavone of banboo leaves extract[J]. Food Science and Technology, 2005(7): 76−79. doi: 10.3969/j.issn.1005-9989.2005.07.023
[16]	Fuerst E P, Okubara P A, Anderson J V, et al. Polyphenol oxidase as a biochemical seed defense mechanism[J/OL]. Frontiers in Plant Science, 2014, 5 (2014−12−05) [2018−10−12]. https://doi.org/10.3389/fpls.2014.00689.
[17]	王光达, 黄初女, 吴委林, 等. 不同玉米品种对大斑病的抗性与相关防御酶活性的关系研究[J]. 玉米科学, 2014, 22(5):146−152. doi: 10.3969/j.issn.1005-0906.2014.05.028 Wang G D, Huang C N, Wu W L, et al. Defense enzyme activities and the resistance to northern leaf blight of different hybrids in maize[J]. Journal of Maize Sciences, 2014, 22(5): 146−152. doi: 10.3969/j.issn.1005-0906.2014.05.028
[18]	樊艳平, 成小芳, 王宏民, 等. 蛋白酶抑制剂对绿豆象幼虫中肠蛋白酶活性的影响[J]. 应用生态学报, 2018, 29(5):1660−1666. Fan Y P, Cheng X F, Wang H M, et al. Effects of protease inhibitors on the activities of midgut proteases in Callosobruchus chinensis (Linnaeus) larvae[J]. Chinese Journal of Applied Ecology, 2018, 29(5): 1660−1666.
[19]	赵爱平, 孙聪, 展恩玲, 等. 蛋白酶抑制剂对梨小食心虫幼虫中肠蛋白酶活性的影响[J]. 昆虫学报, 2016, 59(10):1069−1078. Zhao A P, Sun C, Zhan E L, et al. Effects of protease inhibitors on the activities of midgut proteases of larval Grapholitha molesta (Lepidoptera: Tortricidae)[J]. Acta Entomologica Sinica, 2016, 59(10): 1069−1078.
[20]	赵微, 尹静, 詹亚光, 等. 温度胁迫对白桦悬浮细胞中三萜积累及防御酶活性的影响[J]. 中国生物工程杂志, 2013, 33(2):34−40. Zhao W, Yin J, Zhan Y G, et al. Effects of temperature stress on triterpene accumulation and defense enzyme activity in suspension cells of Betula platyphylla[J]. China Biotechnology, 2013, 33(2): 34−40.
[21]	鲁艺芳, 石蕾, 严善春. 不同光照强度对兴安落叶松几种主要防御蛋白活力的影响[J]. 生态学报, 2012, 32(11):3621−3627. Lu Y F, Shi L, Yan S C. Effects of different light intensities on activities of the primary defense proteins in needles of Larix gmelinii[J]. Acta Ecologica Sinica, 2012, 32(11): 3621−3627.
[22]	Jiang D, Wang Y Y, Dong X W, et al. Inducible defense responses in Populus alba berolinensis to Pb stress[J]. South African Journal of Botany, 2018, 119: 295−300. doi: 10.1016/j.sajb.2018.09.030
[23]	郭光艳, 柏峰, 刘伟, 等. 转录因子对木质素生物合成调控的研究进展[J]. 中国农业科学, 2015, 48(7):1277−1287. Guo G Y, Bo F, Liu W, et al. Advances in research of the regulation of transcription factors of lignin biosynthesis[J]. Scientia Agricultura Sinica, 2015, 48(7): 1277−1287.
[24]	诸姮, 胡宏友, 卢昌义, 等. 植物体内的黄酮类化合物代谢及其调控研究进展[J]. 厦门大学学报(自然科学版), 2007, 46(增刊1): 136−143. Zhu H, Hu H Y, Lu C Y, et al. Advances in the Study of flavonoids metabolism and regulation in plants[J]. Journal of Xiamen University (Natural Science), 2007, 46(Suppl. 1): 136−143.
[25]	张凯, 徐波, 孟昭军, 等. 铜、镉胁迫对杨树叶片中防御蛋白活性的影响[J]. 东北林业大学学报, 2014, 42(11):43−46. doi: 10.3969/j.issn.1000-5382.2014.11.011 Zhang K, Xu B, Meng Z J, et al. Effect of copper and cadmium on defensive protein activity in poplar leaves[J]. Journal of Northeast Forestry University, 2014, 42(11): 43−46. doi: 10.3969/j.issn.1000-5382.2014.11.011
[26]	姜礅, 王月月, 严善春. 锌胁迫对银中杨生长发育和化学防御的影响[J]. 北京林业大学学报, 2018, 40(11):42−48. Jiang D, Wang Y Y, Yan S C. Effects of Zn stress on growth development and chemical defense of Populus alba ‘Berolinensis’ seedlings[J]. Journal of Beijing Forestry University, 2018, 40(11): 42−48.
[27]	Jiang D, Yan S. Effects of Cd, Zn or Pb stress in Populus alba ‘Berolinensis’ on the development and reproduction of Lymantria dispar[J]. Ecotoxicology, 2017, 26(1): 1−9. doi: 10.1007/s10646-016-1742-7
[28]	张凯, 周艳涛, 王皙玮, 等. 铜、镉胁迫对舞毒蛾排毒代谢酶的影响[J]. 北京林业大学学报, 2016, 38(2):61−67. Zhang K, Zhou Y T, Wang X W, et al. Effects of copper and cadmium stress on the activities of detoxifying metabolic enzymes in Lymantria dispar[J]. Journal of Beijing Forestry University, 2016, 38(2): 61−67.
[29]	姜礅, 王月月, 严善春. 银中杨各部位对Cd、Zn、Pb的富集特性[J]. 北京林业大学学报, 2018, 40(1):83−88. Jiang D, Wang Y Y, Yan S C. Accumulation characteristics in all parts of Populus alba ‘Berolinensis ’ to cadmium, zinc, and lead[J]. Journal of Beijing Forestry University, 2018, 40(1): 83−88.

[1]	Jiang Hong, Meng Zhaojun, Li Peng, Xue Yi, Jiang Dun, Li Guojiang, Yan Shanchun. Effects of banding mixed forest on activity of defensive proteins in Larix olgensis[J]. Journal of Beijing Forestry University, 2022, 44(11): 82-89. DOI: 10.12171/j.1000-1522.20210249
[2]	Wu Shuai, Jiang Dun, Ma Qinghui, Tan Mingtao, Zhao Jiaqi, Liu Xiaoxia, Meng Zhaojun, Yan Shanchun. Effects of arbuscular mycorrhizal fungi on metabolism and chemical defense of Populus alba × P. berolinensis leaves[J]. Journal of Beijing Forestry University, 2021, 43(5): 86-92. DOI: 10.12171/j.1000-1522.20200172
[3]	Jiang Dun, Wang Yueyue, Yan Shanchun. Effects of Zn stress on growth development and chemical defense of Populus alba 'berolinensis' seedlings[J]. Journal of Beijing Forestry University, 2018, 40(11): 42-48. DOI: 10.13332/j.1000-1522.20180131
[4]	Jiang Dun, Wang Yue-yue, Yan Shan-chun. Accumulation characteristics in all parts of Populus alba 'Berolinensis' to cadmium, zinc, and lead[J]. Journal of Beijing Forestry University, 2018, 40(1): 83-88. DOI: 10.13332/j.1000-1522.20170191
[5]	JIANG Dun, MENG Zhao-jun, YAN Shan-chun. Effects of partially spraying Larix olgensis seedlings with exogenous methyl jasmonate on the defensive enzyme activities of Dendrolimus superans larvae[J]. Journal of Beijing Forestry University, 2017, 39(2): 58-63. DOI: 10.13332/j.1000-1522.20160291
[6]	ZHANG Rui-bin, HU Xiao, LIU Tong, YAN Shan-chun. Effects of sex, DBH, and needle age of Taxus cuspidata on the activities of the needle defensive proteins[J]. Journal of Beijing Forestry University, 2015, 37(8): 48-52. DOI: 10.13332/j.1000-1522.20140115
[7]	LIU Yu-jie, MAN Xiu-ling. Morphology of soil Zn and Mn in main forest types and potential ecological risk in the Great Xing'an Mountains[J]. Journal of Beijing Forestry University, 2015, 37(7): 45-52. DOI: 10.13332/j.1000-1522.20140482
[8]	YAN Jun-xin, CHI De-fu, ZHANG Yong-qiang2, PANG Hai-yu. Effects of JA and SA on the growth and development as well as defensive enzyme activity of Rosa rugosa.[J]. Journal of Beijing Forestry University, 2013, 35(3): 128-136.
[9]	DONG Yan-he, WANG Cheng-zhang, YE Jian-zhong, ZHOU Hao. Extraction process and chemical constituents of lacquer wax.[J]. Journal of Beijing Forestry University, 2010, 32(4): 256-260.
[10]	SUN De-zhi, , HUANG Xin-rui, CHENG Xiang, CHEN Ai-yan. Phosphate adsorption from sewage sludge filtrate by ZnAl hydrotacitelike compounds. [J]. Journal of Beijing Forestry University, 2009, 31(2): 128-132.

Cited By

Cited by

Periodical cited type(4)

1.	张月莹，蒋丽伟，王焱，李宗伦. 北京明长城沿线森林景观斑块地形梯度分布特征. 绿色科技. 2023(12): 1-7+12 .
2.	李国庆，赵洪升，张浩东. 淮北市林地景观格局特征与优化研究. 绿色科技. 2023(19): 96-100 .
3.	张磊，姜锡川，董运斋. 青岛生态间隔区规划建设初探. 绿色科技. 2022(01): 132-136 .
4.	武田艳，宋璐璐，梁坤宇. 基于SA算法的应急公共服务设施布局优化研究——以上海市长宁区应急避难场所为例. 数学的实践与认识. 2018(24): 32-40 .

Other cited types(7)

Get Citation

PDF

XML

Article views (2004) PDF downloads (22) Cited by(11)

Turn off MathJax

Article Contents

Abstract

References

Effects of Cd-Zn combined stress on chemical defense of Populus alba ‘Berolinensis’