Effects of Zn stress on growth development and chemical defense of <i>Populus alba</i> 'berolinensis' seedlings

Jiang Dun; Wang Yueyue; Yan Shanchun

doi:10.13332/j.1000-1522.20180131

Journal of Beijing Forestry University > 2018 > 40(11): 42-48. > DOI: 10.13332/j.1000-1522.20180131

Jiang Dun, Wang Yueyue, Yan Shanchun. Effects of Zn stress on growth development and chemical defense of Populus alba 'berolinensis' seedlings[J]. Journal of Beijing Forestry University, 2018, 40(11): 42-48. DOI: 10.13332/j.1000-1522.20180131

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Effects of Zn stress on growth development and chemical defense of Populus alba 'berolinensis' seedlings

School of Forestry, Northeast Forestry University, Harbin 150040, Heilongjiang, China

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Received Date: April 26, 2018
Revised Date: September 27, 2018
Published Date: October 31, 2018

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Abstract

Abstract

ObjectiveThis study aims to investigate the effects of Zn stress on growth development of Populus alba 'berolinensis' (PAB) seedlings and their chemical defense patterns in response to the Zn stress.
MethodWe analyzed the growth development status, nutrient content and secondary metabolites, as well as the protease inhibitor activities in the leaves of PAB seedlings growing in either non-contaminated soil (CK/control) or Zn-contaminated soil (300, 500 and 700 mg/kg).
ResultOur results showed that the growth parameters (plant height, root length and ground diameter) and biomass parameters (the fresh and dry mass of root, stem and leaf) of the PAB seedlings in all the Zn-treated groups were significantly lower than those in the control (P < 0.05) in a concentration/dose-dependent fashion. On the 30th, 40th and 50th days after Zn stress, the contents of proteins and soluble sugars in PAB leaves in all the Zn-treated groups were significantly lower than those in control (P < 0.05). There were no significant differences in protein contents between the Zn treatment groups (P>0.05), while soluble sugar contents increased with the increase of Zn concentration. Zn stress, regardless of its concentration level, significantly increased the contents of flavonoids and lignin in the leaves on all three sampling dates compared with the control, whereas the trypsin inhibitor (TI) and the chymotrypsin inhibitor (CI) activities were significantly decreased (P < 0.05).
ConclusionThe above results illustrate that excessive Zn in the growing environment may inhibit the growth of PAB seedlings and affect their chemical defense. The response patterns of nutrient substances, secondary metabolites related to the plant resistance to insects as well as the protease inhibitors in the PAB leaves to Zn stress are inconsistent, as was evidenced by the reduced nutrient contents, the increased secondary metabolite contents and the weakened protease inhibitor activities.
- Zn stress,
- Populus alba 'berolinensis',
- growth development,
- chemical defense

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Effects of Zn stress on growth development and chemical defense of Populus alba 'berolinensis' seedlings