沙冬青胚胎晚期发生丰富蛋白基因序列及表达特性分析
Sequence analysis and expression pattern of AmLEA14 encoding a late embryogenesis abundant protein in Ammopiptanthus mongolicus．
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摘要: 从沙冬青叶片EST文库中获得了胚胎晚期发生丰富蛋白（LEA）基因cDNA全长序列，命名为AmLEA14。序列分析表明该基因全长579 bp，含有一个459 bp编码152个氨基酸的开放阅读框，推测编码的蛋白质分子质量为166 ku。二级结构预测显示此编码蛋白属于第4类LEA蛋白。系统发生分析表明，此编码蛋白与大豆LEA14蛋白（P465191）的亲缘关系最近。荧光定量PCR结果显示，AmLEA14的表达量在低温、干旱、盐胁迫条件下升高，但是主要在低温胁迫后期富集。推测AmLEA14基因可能在沙冬青抵御低温、干旱、盐胁迫中发挥作用，主要是参与沙冬青的低温防御机制。
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关键词:
- 沙冬青 /
- 胚胎晚期发生丰富蛋白 /
- 序列分析 /
- 表达分析
Abstract: For a better understanding of stress responses of desert plant Ammopiptanthus mongolicus, we reported molecular characterization and expression analysis of AmLEA14 gene from the EST data of cold and droughtstressed A. mongolicus. Sequence analysis showed that the overall length of gene AmLEA14 was 579 bp, and the cDNA of AmLEA14 contained a 459 bp ORF encoding a polypeptide of 152 amio acids with a calculated molecular mass of 166 kDa and a theoretical pI of 49. Sequence and structural analysis showed that AmLEA14 protein was a member of late embryogenesis abundant protein class 4. Phylogenetic analysis indicated that AmLEA14 protein was most closely related to soybean LEA14 protein (P465191). Realtime quantitative PCR analysis showed that AmLEA14 was constitutively expressed, upregulated by cold, drought stressed, and specifically accumulated at the late stage of cold treatment. Otherwise, AmLEA14 was downregulated by heat stress. The combined results indicate that AmLEA14 protein plays some role in abiotic stress responses, mainly in cold tolerant mechanism, and is valuable for future abiotic stress researches in A. mongolicus. -
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期刊类型引用(9)
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百度学术
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5. 张林华，刘超，邱红林，王爱英，邓福军，祝建波. 天山雪莲LEA14基因克隆及功能分析. 西北植物学报. 2013(06): 1071-1078 . 百度学术
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