Characteristics of <i>PwUSP</i>1 in <i>Picea wilsonii</i> and its response to drought and salt stress

Liu Junling; Liang Kehao; Miao Yahui; Hu Anni; Sun Yongjiang; Zhang Lingyun

doi:10.12171/j.1000-1522.20200063

Journal of Beijing Forestry University > 2020 > 42(10): 62-70. > DOI: 10.12171/j.1000-1522.20200063

Liu Junling, Liang Kehao, Miao Yahui, Hu Anni, Sun Yongjiang, Zhang Lingyun. Characteristics of PwUSP1 in Picea wilsonii and its response to drought and salt stress[J]. Journal of Beijing Forestry University, 2020, 42(10): 62-70. DOI: 10.12171/j.1000-1522.20200063

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Characteristics of PwUSP1 in Picea wilsonii and its response to drought and salt stress

School of Forestry, State Key Laboratory of Forest Cultivation and Protection of Ministry of Education, Beijing Forestry University, Beijing 100083, China

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Received Date: March 08, 2020
Revised Date: April 19, 2020
Available Online: October 01, 2020
Published Date: October 24, 2020

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Abstract

Abstract

Objective Universal stress proteins (USP) are stress-related genes which are widely reported to participate in the process of abiotic-stress response of plants. By identifying and validating the function of PwUSP1, we revealed the role of PwUSP1 in plants under drought and salt stress, thereby providing candidate genes for improving the tolerance to abiotic stress through genetic engineering in Picea wilsonii.
Method Transient transformation of tobacco leaves was used to reveal the location of PwUSP1 in cells. Yeast two-hybrid experiment was used to determine whether PwUSP1 could form homodimers by itself. The Arabidopsis Col-0 (WT) was transferred by floral dip method to obtain homozygous PwUSP1 overexpression lines. The survival rates and water loss rates of PwUSP1 overexpression plants, wild type (WT) and empty vector (VC) were measured to analyze and compare the tolerance of different lines when plants were subjected to drought and salinity. DAB and NBT staining, the activities of SOD, POD, CAT and the content of MDA were determined to explore the potential physiological mechanism of PwUSP1 acting.
Result Subcellular localization results showed that PwUSP1 was located in the nucleus, cytoplasm and cell membrane. Besides, the yeast two hybrid experiment showed that PwUSP1 itself can form homodimers. qRT-PCR was used to detect transgenic Arabidopsis, and the results showed that two independent homozygous lines with stable overexpression (L1, L7) were successfully obtained for further analysis. Under drought or salt stress, compared with WT and VC, PwUSP1 overexpression lines significantly improved the drought or salt tolerance of plants, and showed higher survival rates and lower water loss rates. Furthermore, overexpression of PwUSP1 obviously reduced the content of H₂O₂ and O₂ ⁻, and simultaneously promoted the activities of antioxidant enzymes and inhibited the accumulation of MDA.
Conclusion PwUSP1 is located in the nucleus, cytoplasm and cell membrane, and can form homodimers by itself. Under drought or salt stress, PwUSP1 improves the tolerance to abiotic stress of plants by enhancing ROS scavenging ability and inhibiting membrane lipid peroxidation.
- Picea wilsonii,
- PwUSP1,
- drought stress,
- salt stress,
- ROS scavenging

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References

[1]	Van Bogelen R A, Hutton M E, Neidhardt F C. Gene-protein database of escherichia coli K-12: edition 3[J]. Electrophoresis, 1990, 11(12): 1131−1166. doi: 10.1002/elps.1150111205.
[2]	Kvint K, Nachin L, Diez A, et al. The bacterial universal stress protein: function and regulation[J]. Current Opinion in Microbiology, 2003, 6: 140−145.
[3]	Sousa M C, Mckay D B. Structure of the universal stress protein of Haemophilus influenzae[J]. Structure, 2001, 9(12): 1135−1141. doi: 10.1016/S0969-2126(01)00680-3.
[4]	Aravind L, Anantharaman V, Koonin E V. Monophyly of class I aminoacyl tRNA synthetase, USPA, ETFP, photolyase, and PP-ATPase nucleotide-binding domains: implications for protein evolution in the RNA world[J]. Proteins: Structure, Function, and Genetics, 2002, 48(1): 1−14. doi: 10.1002/prot.10064.
[5]	Kerk D, Bulgrien J, Smith D W, et al. Arabidopsis proteins containing similarity to the universal stress protein domain of bacteria[J]. Plant Physiology, 2003, 131(3): 1209−1219. doi: 10.1104/pp.102.016006.
[6]	Diez A, Gustavsson N, Nystrom T. The universal stress protein A of Escherichia coli is required for resistance to DNA damaging agents and is regulated by a RecA/FtsK-dependent regulatory pathway[J]. Molecular Microbiology, 2000, 36(6): 1494−1503.
[7]	Jones P G, Cashel M, Glaser G, et al. Function of a relaxed-like state following temperature downshifts in Escherichia coli[J]. Journal of Bacteriology, 1992, 174(12): 3903−3914. doi: 10.1128/JB.174.12.3903-3914.1992.
[8]	Javeed A, Fan M X, Shi L, et al. Isolation and characterization of a pollen- specific gene Zmstk2_USP from Zea mays[J]. Applied Ecology and Environmental Research, 2018, 16(1): 487−494. doi: 10.15666/aeer/1601_487494.
[9]	Jung Y J, Melencion S M, Lee E S, et al. Universal stress protein exhibits a redox-dependent chaperone function in Arabidopsis and enhances plant tolerance to heat shock and oxidative stress[J]. Frontiers in Plant Science, 2015, 6: 1141−1151.
[10]	Loukehaich R, Wang T, Ouyang B, et al. SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato[J]. Journal of Experimental Botany, 2012, 63(15): 5593−5606. doi: 10.1093/jxb/ers220.
[11]	Sinha P, Pazhamala L T, Singh V K, et al. Identification and validation of selected universal stress protein domain containing drought-responsive genes in Pigeonpea (Cajanus cajan L.)[J]. Frontiers in Plant Science, 2015, 6: 1065−1073.
[12]	Udawat P, Mishra A, Jha B. Heterologous expression of an uncharacterized universal stress protein gene (SbUSP) from the extreme halophyte, Salicornia brachiata, which confers salt and osmotic tolerance to E. coli[J]. Gene, 2014, 536(1): 163−170. doi: 10.1016/j.gene.2013.11.020.
[13]	Bhuria M, Goel P, Kumar S, et al. The promoter of AtUSP is co-regulated by phytohormones and abiotic stresses in Arabidopsis thaliana[J]. Frontiers in Plant Science, 2016, 7: 1957−1969.
[14]	Udawat P, Jha R K, Sinha D, et al. Overexpression of a cytosolic abiotic stress responsive universal stress protein (SbUSP) mitigates salt and osmotic stress in transgenic tobacco plants[J]. Frontiers in Plant Science, 2016, 7: 518−538.
[15]	Li W, Zhao F, Fang W, et al. Identification of early salt stress responsive proteins in seedling roots of upland cotton (Gossypium hirsutum L.) employing iTRAQ-based proteomic technique[J]. Frontiers in Plant Science, 2015, 6: 732−745.
[16]	Maqbool A, Zahur M, Husnain T, et al. GUSP1 and GUSP2, two drought-responsive genes in Gossypium arboreum have homology to universal stress proteins[J]. Plant Molecular Biology Reporter, 2009, 27(1): 109−114. doi: 10.1007/s11105-008-0049-0.
[17]	Chi Y H, Koo S S, Oh H T, et al. The physiological functions of universal stress proteins and their molecular mechanism to protect plants from environmental stresses[J]. Frontiers in Plant Science, 2019, 10: 750−762. doi: 10.3389/fpls.2019.00750.
[18]	游韩莉, 袁义杭, 李长江, 等. 青杄MYB转录因子基因PwMYB20的克隆及表达分析[J]. 林业科学, 2017, 53(5):23−32. doi: 10.11707/j.1001-7488.20170504. You H L, Yuan Y H, Li C J, et al. Cloning and expression analysis of MYB homologous gene PwMYB20 from Picea wilsonii[J]. Scientia Silvae Sinicae, 2017, 53(5): 23−32. doi: 10.11707/j.1001-7488.20170504.
[19]	崔晓燕, 李长江, 孙帆, 等. 青杆PwUSP1基因的克隆及表达模式分析[J]. 植物生理学报, 2014, 50(4):407−414. Cui X Y, Li C J, Sun F, et al. Cloning and expression analysis of PwUSP1 from Picea wilsonii[J]. Plant Physiology Journal, 2014, 50(4): 407−414.
[20]	Clough S J, Bent A F. Floral dip: a simplified method for agrobacterium-mediated transformation of Arabidopsis thaliana[J]. Plant Journal, 1999, 16(6): 735−743.
[21]	Zhang H, Cui X, Guo Y, et al. Picea wilsonii transcription factor NAC2 enhanced plant tolerance to abiotic stress and participated in RFCP1-regulated flowering time[J]. Plant Molecular Biology, 2018, 98(6): 471−493. doi: 10.1007/s11103-018-0792-z.
[22]	Nakashima A, Chen L, Thao N P, et al. RACK1 functions in rice innate immunity by interacting with the Rac1 immune complex[J]. Plant Cell, 2008, 20(8): 2265−2279. doi: 10.1105/tpc.107.054395.
[23]	Melencion S M B, Chi Y H, Pham T T, et al. RNA chaperone function of a universal stress protein in Arabidopsis confers enhanced cold stress tolerance in plants[J]. International Journal of Molecular Sciences, 2017, 18(12): 2546−2561. doi: 10.3390/ijms18122546.
[24]	Nachin L, Brive L, Persson K, et al. Heterodimer formation within universal stress protein classes revealed by an in Silico and experimental approach[J]. Journal of Molecular Biology, 2008, 380(2): 340−350. doi: 10.1016/j.jmb.2008.04.074.
[25]	Yang M, Che S, Zhang Y, et al. Universal stress protein in Malus sieversii confers enhanced drought tolerance[J]. Journal of Plant Research, 2019, 132(6): 825−837. doi: 10.1007/s10265-019-01133-7.
[26]	刘灿. 盐芥TsNAC1转录因子及其在玉米中同源基因功能的研究[D]. 济南: 山东大学, 2018. Liu C. Function analysis of TsNAC1 in T. halophila and its orthologs in Zea mays[D]. Jinan: Shandong University, 2018.
[27]	王晋芳. 黄瓜NAC转录因子CsATAF1响应干旱胁迫的机制研究[D]. 北京: 中国农业大学, 2018. Wang J F. The mechanism of NAC transcription factor CsATAF1 in response to drought stress in cucumber[D]. Beijing: China Agricultural University, 2018.
[28]	Gutiérrez-Beltrán E, Personat J M, Torre F D, et al. A universal stress protein involved in oxidative stress is a phosphorylation target for protein kinase CIPK6[J]. Plant Physiology, 2017, 173(1): 836−852. doi: 10.1104/pp.16.00949

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Characteristics of PwUSP1 in Picea wilsonii and its response to drought and salt stress