Identification of <i>PtNF-YC</i>1 of <i>Pinus tabuliformis</i> and its molecular mechanism involved in regulation of cone development

Liu Hongmei; Zheng Yongtao; Guo Yingtian; Zhang Jingxing; Li Wei

doi:10.12171/j.1000-1522.20220250

Journal of Beijing Forestry University > 2023 > 45(9): 1-8. > DOI: 10.12171/j.1000-1522.20220250

Liu Hongmei, Zheng Yongtao, Guo Yingtian, Zhang Jingxing, Li Wei. Identification of PtNF-YC1 of Pinus tabuliformis and its molecular mechanism involved in regulation of cone development[J]. Journal of Beijing Forestry University, 2023, 45(9): 1-8. DOI: 10.12171/j.1000-1522.20220250

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Identification of PtNF-YC1 of Pinus tabuliformis and its molecular mechanism involved in regulation of cone development

1.
School of Biological Sciences and Biotechnology, National Engineering Laboratory of Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, Beijing Forestry University, Beijing 100083, China
2.
National Beipiao Daqingshan Forest Farm, Beipiao 122100, Liaoning, China

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Received Date: June 20, 2022
Revised Date: August 15, 2022
Available Online: June 29, 2023
Published Date: September 24, 2023

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Abstract

Abstract

Objective The research on the regulation of NF-Y nuclear factor on cone development in conifers has not been reported yet. Through the cloning, expression characteristics and functional analysis of PtNF-YC1 gene of Pinus tabuliformis, it provides a basis for the functional study of conifer NF-Y gene family in the reproductive development of conifers.
Method (1) The relationship between PtNF-YC1 and Arabidopsis thaliana NF-YC subfamily proteins was analyzed by phylogenetic tree. (2) Tobacco was transiently transformed to detect the subcellular localization of PtNF-YC1. (3) The expression characteristics of PtNF-YC1 in different tissues of P. tabuliformis were analyzed based on transcriptome data. (4) PtNF-YC1 was heterologously transformed into Arabidopsis thaliana , and the flowering time of different transgenic Arabidopsis thaliana lines under long-day and short-day was compared. The transcriptome sequencing of each transgenic line under long-day was performed to screen the genes related to PtNF-YC1 regulating flowering. (5) The protein interaction between PtNF-YC1 and candidate proteins was verified by Y2H and BiFC.
Result The open reading frame of PtNF-YC1 was 897 bp, which encoded 299 amino acids, had a typical NF-YC conserved domain, and a close relationship with the homologous genes of AtNF-YC3/4/9. The subcellular localization showed that PtNF-YC1 was localized in the nucleus and cytoplasm. The analysis of expression patterns in different tissues showed that PtNF-YC1 could be expressed in needles, vegetative buds, male and female cones and roots, but the expression abundance was the highest in male cones and stems. PtNF-YC1 heterologous transformation of Arabidopsis thaliana delayed flowering under short day. It was proved that PtNF-YC1 interacted with PtCOL5 through Y2H and BiFC.
Conclusion PtNF-YC1 has the function of regulating flowering time and is a candidate gene for inducing cone development through photoperiodic pathway of P. tabuliformis.
- Pinus tabuliformis,
- PtNF-YC1,
- photoperiod regulation,
- cone development

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References (31)

References

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