Inhibitors during cutting process of ancient <i>Platycladus orientalis</i>  trees of different ages

Wei Qianchun; Liu Jianfeng; Guo Wei; Wang Hezhi; Xu Jiannan; Chao Bixiao; Chang Ermei

doi:10.12171/j.1000-1522.20230045

Journal of Beijing Forestry University > 2024 > 46(11): 83-91. > DOI: 10.12171/j.1000-1522.20230045

Wei Qianchun, Liu Jianfeng, Guo Wei, Wang Hezhi, Xu Jiannan, Chao Bixiao, Chang Ermei. Inhibitors during cutting process of ancient Platycladus orientalis trees of different ages[J]. Journal of Beijing Forestry University, 2024, 46(11): 83-91. DOI: 10.12171/j.1000-1522.20230045

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Inhibitors during cutting process of ancient Platycladus orientalis trees of different ages

1.
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
2.
Taishan Academy of Forestry Sciences, Taian 271000, Shandong, China
3.
Academy of Inventory and Planning, National Forestry and Glassland Administration, Beijing 100714, China

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Received Date: March 05, 2023
Revised Date: May 28, 2023
Available Online: November 12, 2024

Graphical Abstract

Abstract

Abstract

Objective
This paper explores the effects of endogenous inhibitors (such as phenolic acids and flavonoids) produced by ancient Platycladus orientalis trees of different ages on rooting during the cutting process, so as to provide a theoretical basis for improving the rooting rate of ancient P. orientalis cuttings.
Method
The cuttings from 5-, 100-, 300-, and 700-year-old P. orientalis were used as experimental materials for cutting propagation. The effects of extracts from the cuttings of P. orientalis of different ages (5-, 100-, 300-, and 700-year-old) in March and June on the germination of cabbage seeds were studied, and the contents of phenolic acids and flavonoids in the cuttings were measured to compare the effects of phenolic acids and flavonoids produced during the rooting process of cuttings of P. orientalis trees of different ages from June on the rooting process. Screening different removal methods was to identify the optimal approach for eliminating inhibitors from 100-year-old P. orientalis cuttings.
Result
(1) The rooting rates and root numbers of ancient P. orientalis trees (100-, 300-, and 700-year-old) were significantly lower than those of young P. orientalis trees (5-year-old ) (P < 0.05). (2) The inhibitory effects of extracts from 100-, 300-, and 700-year-old P. orientalis cuttings on cabbage seed germination in March were significantly higher than that in June (P < 0.05). As the ages of trees increased, the inhibitory effects of extracts of cuttings on inhibiting the germination of cabbage seeds increased. (3) The contents of phenolic acids and flavonoids in P. orientalis cuttings from June were generally low. In the experimental materials from March and June, the contents of rutin, salicylic acid, gallic acid, coumaric acid, and ferulic acid generally showed an increasing trend with the increase of age of P. orientalis. In the experimental materials from June, the phenolic acid and flavonoid contents of 5-, 100-, 300-, and 700-year-old P. orientalis cuttings showed an increasing trend with the development of rooting process. (4) Treating cuttings of ancient P. orientalis with 0.1% silver nitrate solution can promote the formation of adventitious roots, and treating them with 0.05% potassium permanganate solution can promote root development.
Conclusion
As the age of P. orientalis grows, the phenolic acid and flavonoid contents of cuttings increase, which have inhibitory effects on the formation of adventitious roots. However, treatment of P. orientalis cuttings with 0.1% silver nitrate or 0.05% potassium permanganate can effectively alleviate this inhibitory effect and improve the rooting rates of P. orientalis cuttings. The study provides theoretical basis and technical support for improving rooting rates.
- Platycladus orientalis,
- ancient tree,
- cuttings,
- inhibitors,
- rooting

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References (29)

References

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Inhibitors during cutting process of ancient Platycladus orientalis trees of different ages