Analysis and evaluation of triterpenoids in tetraploid birch (Betula).

WANG Sui; ZHAO Hui; YANG Chuan-ping; JIANG Jing.

doi:10.13332/j.1000-1522.20140150

Journal of Beijing Forestry University > 2015 > 37(9): 53-61. > DOI: 10.13332/j.1000-1522.20140150

WANG Sui, ZHAO Hui, YANG Chuan-ping, JIANG Jing.. Analysis and evaluation of triterpenoids in tetraploid birch (Betula).[J]. Journal of Beijing Forestry University, 2015, 37(9): 53-61. DOI: 10.13332/j.1000-1522.20140150

Citation:

PDF (997 KB)

Analysis and evaluation of triterpenoids in tetraploid birch (Betula).

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, Heilongjiang, 150040, P.R. China.

More Information

Received Date: April 26, 2014
Published Date: September 29, 2015

Graphical Abstract

Abstract

Abstract

Betulinic acid, oleanolic acid and betulin are three pentacyclic triterpenoids in birch bark with high contents and a variety of medicinal values. In this study, we used ultrasonic-assistant measure to extract triterpenoids from birch bark rapidly with high efficiency. Using HPLC, we separated and detected the three kinds of triterpenoids. Then, we examined the differences in contents of three triterpenoids between two kinds of birches by building violin plots and star plots. Finally, we applied factor analysis method to screen the tetraploid birches with excellent comprehensive traits. The HPLC assay was performed using a reversed-phase C18 column (4.6 mm 250 mm 5 m) with a column temperature of 30 ℃. The mobile phase was composed of acetonitrile and 0.1% aqueous phosphoric acid with a flow rate of 0.5 mL/min and detection wavelength of 195 nm. There were significant differences in contents of betulinic acid and betulin between the two kinds of tetraploid birches at the confidence level of 0.10, and also a significant difference in oleanolic acid content at the confidence level of 0.05. The contents of three triterpenoids in B. platyphylla B. pendula were always higher than that in B. platyphylla. The results indicated that there was a significantly positive correlation among contents of three triterpenoids. Analysis revealed that the correlation coefficient was the highest (0.911) between betulinic acid and betulin and the lowest (0.631) between oleanolic acid and betulin. With factor analysis we obtained the following comprehensive evaluation formula for individual birch plants:F=48.620%F1 + 21.080%F2 + 21.075%F3, and screened out the individual plants with excellent comprehensive characters. The three top-scorer individuals of B. platyphylla were Nos. 4126, 4123 and 4125, and those of B. platyphylla B. pendula were Nos. 4202, 4204 and 4208.
- Betula,
- tetraploid,
- betulinic acid,
- oleanolic acid,
- betulin,
- violin plot,
- star plot,
- factor analysis

FullText(HTML)

References (30)

References

[1]	ZHANG Z X. Dendrology (North edition) [M]. Beijing: China Forestry Publishing House, 2008: 195-197.
[1]	张志翔. 树木学(北方本)[M]. 北京: 中国林业出版社, 2008: 195-197.
[2]	KRASUTSKY P A. Birch bark research and development[J]. Natural Product Reports, 2006, 23(6): 919-942.
[3]	ALAKURTTI S, M KEL T, KOSKIMIES S, et al. Pharmacological properties of the ubiquitous natural product betulin[J]. European Journal of Pharmaceutical Sciences, 2006, 29(1): 1-13.
[4]	DEHELEAN C A, SOICA C, LEDETI I, et al. Study of the betulin enriched birch bark extracts effects on human carcinoma cells and ear inflammation[J]. Chem Cent J, 2012, 6(1): 137.
[5]	FUJIOKA T, KASHIWADA Y, KILKUSKIE R E, et al.Anti-AIDS agents, 11. Betulinic acid and platanic acid as anti-HIV principles from Syzigium claviflorum, and the anti-HIV activity of structurally related triterpenoids[J]. J Nat Prod, 1994, 57(2): 243-247.
[6]	PISHA E, CHAI H, LEE I S, et al. Discovery of betulinic acid as a selective inhibitor of human melanoma that functions by induction of apoptosis[J]. Nat Med, 1995, 1(10): 1046-1051.
[7]	FU J Y, QIAN L B, ZHU L G, et al. Betulinic acid ameliorates endothelium-dependent relaxation in L-NAME-induced hypertensive rats by reducing oxidative stress[J]. Eur J Pharm Sci, 2011, 44(3): 385-391.
[8]	LIU J. Pharmacology of oleanolic acid and ursolic acid[J]. J Ethnopharmacol, 1995, 49(2): 57-68.
[9]	POLLIER J, GOOSSENS A. Oleanolic acid[J]. Phytochemistry, 2012, 77: 10-15.
[10]	刘福妹,穆怀志,刘子嘉,等. 用秋水仙素处理不同家系白桦种子诱导四倍体的研究[J]. 北京林业大学学报, 2013,35(3): 84-89.
[11]	LIU F M, MU H Z, LIU Z J, et al. Inducing tetraploid of Betula platyphylla with different generations of seeds by colchicine[J]. Journal of Beijing Forestry University, 2013,35(3): 84-89.
[12]	穆怀志. 白桦四倍体的生长生殖特征及基因转录组分析[D]. 哈尔滨: 东北林业大学, 2013.
[13]	MU H Z. Transcriptomic analysis of growth and reproductive changes in birch (Betula platyphylla) autotetraploids[D]. Harbin: Northeast Forestry University, 2013.
[14]	DHAWAN O P, LAVANIA U C. Enhancing the productivity of secondary metabolites via induced polyploidy: a review[J]. Euphytica, 1996, 87(2): 81-89.
[15]	KIM Y, HAHN E, MURTHY H N, et al. Effect of polyploidy induction on biomass and ginsenoside accumulations in adventitious roots of ginseng[J]. Journal of Plant Biology, 2004, 47(4): 356-360.
[16]	骆沙曼,丁为民,于涛,等. 桦木醇的液泛提取工艺研究[J]. 林产化学与工业, 2013, 33(1): 54-58.
[17]	LUO S M, DING W M, YU T, et al. Floodingextraction of betulin[J]. Chemistry and Industry of Forest Products, 2013, 33(1): 54-58.
[18]	KROMIDAS S, KUSS H J.Quantification in LC and GC: a practical guide to good chromatographic data[M]. Hoboken: John Wiley Sons, 2009: 190-214.
[19]	GUO S, DUAN J, TANG Y, et al. High-performance liquid chromatography: two wavelength detection of triterpenoid acids from the fruits of Ziziphus jujuba containing various cultivars in different regions and classification using chemometric analysis[J]. Journal of Pharmaceutical and Biomedical Analysis, 2009, 49(5): 1296-1302.
[20]	HINTZE J L, NELSON R D. Violin plots: a box plot-density trace synergism[J]. The American Statistician, 1998, 52(2): 181-184.
[21]	BECKER R A, CHAMBERS J M, WILKS A R. The new S language[M]. Pacific Grove: Wadsworth Brooks/Cole, 1988.
[22]	WU A R, NEFF N F, KALISKY T, et al. Quantitative assessment of single-cell RNA-sequencing methods[J]. Nat Methods, 2014, 11(1): 41-46.
[23]	CARON J B, GAINES R R, ARIA C, et al. A new phyllopod bed-like assemblage from the Burgess shale of the Canadian Rockies[J]. Nat Commun, 2014, 5: 3210.
[24]	TREUTLEIN B, BROWNFIELD D G, WU A R, et al. Reconstructing lineage hierarchies of the distal lung epithelium using single-cell RNA-seq[J]. Nature, 2014, 509: 371-375.
[25]	SPITZER M, WILDENHAIN J, RAPPSILBER J, et al. BoxPlotR: a web tool for generation of box plots[J]. Nat Methods, 2014, 11(2): 121-122.
[26]	MADLUNG A. Polyploidy and its effect on evolutionary success: old questions revisited with new tools[J]. Heredity, 2013, 110(2): 99-104.
[27]	DE JESUS-GONZALEZ L, WEATHERS P J. Tetraploid Artemisia annua hairy roots produce more artemisinin than diploids[J]. Plant Cell Reports, 2003, 21(8): 809-813.
[28]	CARUSO I, LEPORE L, DE TOMMASI N, et al. Secondary metabolite profile in induced tetraploids of wild Solanum commersonii Dun.[J]. Chem Biodivers, 2011, 8(12): 2226-2237.
[29]	HUANG L, LI J, YE H, et al. Molecular characterization of the pentacyclic triterpenoid biosynthetic pathway in Catharanthus roseus[J]. Planta, 2012, 236(5): 1571-1581.

[1]	Wu Fei, Gao Zhangwei, Zhang Ruibo, Shi Rongxi, Liu Mengjie, Hu Jian, Wang Hui, Zhou Qingping. Effects of warming on soil microbial diversity and functional potential in alpine meadows[J]. Journal of Beijing Forestry University, 2025, 47(1): 29-38. DOI: 10.12171/j.1000-1522.20240064
[2]	Liu Yuxiang, Wang Lei, Ren Ninghong, You Chongjuan. Community structure of endophytic fungi in asymptomatic and symptomatic Pinus sylvestris var. mongolica infected by diplodia tip blight[J]. Journal of Beijing Forestry University, 2024, 46(9): 119-131. DOI: 10.12171/j.1000-1522.20230350
[3]	Wang Xuelin, Liu Jinfu, He Zhongsheng, Wu Zeyan, Jiang Lan, Zhu Jing, Xing Cong, Gu Xinguang. Seasonal dynamics of functional diversity of soil microbial communities in Castanopsis kawakamii forest gaps[J]. Journal of Beijing Forestry University, 2020, 42(7): 77-88. DOI: 10.12171/j.1000-1522.20200052
[4]	Zeng Ming, He Shuhang, Li Wenhai, Feng Jun, Zhao Yuanyuan, Zheng Caixia. Differential expression of miRNA and function of target genes in heteromorphic leaves of Populus euphratica[J]. Journal of Beijing Forestry University, 2020, 42(6): 1-13. DOI: 10.12171/j.1000-1522.20190464
[5]	Deng Jiaojiao, Zhou Yongbin, Yin You, Bai Xuejiao, Gao Huilin, Zhu Wenxu. Characteristics of soil fungal community structure at two coniferous plantations in mountainous region of eastern Liaoning Province, northeastern China[J]. Journal of Beijing Forestry University, 2019, 41(9): 130-138. DOI: 10.13332/j.1000-1522.20180147
[6]	Jiang Jun, Liu Xianzhao, Jia Hongyan, Ming Angang, Chen Beibei, Lu Yuanchang. Effects of stand density on understory species diversity and soil physicochemical properties after close-to-nature transformation management of Chinese fir plantation[J]. Journal of Beijing Forestry University, 2019, 41(5): 170-177. DOI: 10.13332/j.1000-1522.20190022
[7]	Fan Zhouzhou, Lu Shuyu, Wang Jiao, Guo Dongchu, Pang Danbo, Zhou Jinxing, Peng Xiawei. Microbial and enzyme activities in rhizosphere soil of different forest stand in karst and non karst areas[J]. Journal of Beijing Forestry University, 2018, 40(7): 55-61. DOI: 10.13332/j.1000-1522.20180011
[8]	Lu Mei, Sun Xiangyang, Tian Kun, Ren Yulian, Wang Shaojun, Wang Hang, Peng Shuxian. Characteristics of soil fungal community structure at different degraded stages in Napahai Plateau Wetland of northwestern China[J]. Journal of Beijing Forestry University, 2018, 40(3): 55-65. DOI: 10.13332/j.1000-1522.20170424
[9]	LIU Hai-yan, WEI Tian-xing, WANG Xian. Soil microbial community structure and functional diversity in typical plantations marked by PLFA in hilly loess region[J]. Journal of Beijing Forestry University, 2016, 38(1): 28-35. DOI: 10.13332/j.1000--1522.20150262
[10]	XU Qiu-fang, JIANG Pei-kun, WANG Qi-zan, LU Yi-tong. Effects of green manure on soil microbial properties of Phyllostachys pubescens stands under intensive management.[J]. Journal of Beijing Forestry University, 2009, 31(6): 43-48.

Cited By

Cited by

Periodical cited type(0)

Other cited types(1)

Get Citation

PDF

XML

Article views (1748) PDF downloads (23) Cited by(1)

Turn off MathJax

Article Contents

Abstract

References

Analysis and evaluation of triterpenoids in tetraploid birch (Betula).

Abstract

References

Related Articles

Cited by

Periodical cited type(0)

Other cited types(1)

Catalog

Analysis and evaluation of triterpenoids in tetraploid birch (Betula).

Abstract

References

Related Articles

Cited by

Periodical cited type(0)

Other cited types(1)

Catalog

Export File

Citation

Format

Content