Effects of copper and cadmium stress on the activities of detoxifying metabolic enzymes in Lymantria dispar

ZHANG Kai; ZHOU Yan-tao; WANG Xi-wei; JIANG Hong; WANG Jia-bing; ZHANG Wen-yi; YAN Shan-chun

doi:10.13332/j.1000-1522.20150293

Journal of Beijing Forestry University > 2016 > 38(2): 61-67. > DOI: 10.13332/j.1000-1522.20150293

ZHANG Kai, ZHOU Yan-tao, WANG Xi-wei, JIANG Hong, WANG Jia-bing, ZHANG Wen-yi, YAN Shan-chun. Effects of copper and cadmium stress on the activities of detoxifying metabolic enzymes in Lymantria dispar[J]. Journal of Beijing Forestry University, 2016, 38(2): 61-67. DOI: 10.13332/j.1000-1522.20150293

Citation:

PDF (988 KB)

Effects of copper and cadmium stress on the activities of detoxifying metabolic enzymes in Lymantria dispar

1.
1 College of Forestry, Northeast Forestry University, Harbin, Heilongjiang, 150040, P. R. China;
2.
2 Changchun Landscape Plant Protection Station, Changchun, Jilin, 130022, P. R. China;
3.
3 General Station of Forest Pest Management, State Forestry Administration, Shenyang, Liaoning, 110034, P. R. China

More Information

Received Date: August 06, 2015
Revised Date: October 21, 2015
Published Date: February 28, 2016

Graphical Abstract

Abstract

Abstract

In order to investigate the changes of detoxification metabolic activity in gypsy moth (Lymantria dispar) under the heavy metal pollution stress, we measured the detoxifying metabolism in gypsy moth body feeding on one-year potted seedlings of Populus simonii×P. nigra after treating the soil with three concentrations of copper (Cu, 400, 700, 1 000 mg/kg) and cadmium (Cd, 1.0, 3.0, 6.0 mg/kg). The results showed that Cu and Cd had a significant impact on the detoxification metabolic enzyme in the third and fourth instar larvae. The activities of SOD, ACP and AKP enzymes in vivo in the third instar larvae at medium and high concentrations of Cu and Cd were lower than the control group (P0.05),but at the low concentrations of Cu and Cd, the difference was not obvious (P0.05). The change of activities of POD and GSTs was just the opposite. The activities of POD and GSTs enzymes in the third instar larvae at medium and high concentrations of Cu and Cd increased significantly (P0.05), but not obvious (P0.05) at the low concentrations of Cu and Cd. The activities of SOD, POD, ACP, AKP and GSTs enzymes in the fourth instar larvae at medium and high concentrations of Cu and Cd decreased significantly (P0.05),but not obvious at the low concentrations of Cu and Cd treatment (P0.05). These results showed that the high levels of Cu and Cd stress could significantly interfere with the metabolic detoxification of gypsy moth larvae, and with the third instar larvae developing into the fourth instar, the heavy metal stress finally showed a significant inhibition to the development of larvae, while low levels of Cu and Cd stress had not significant interference with metabolic detoxification of the larvae.
- heavy metal stress,
- Populus simonii×P. nigra,
- Lymantria dispar,
- detoxifying metabolic enzyme

FullText(HTML)

References (44)

References

[1]	GAO M, JIN L, GUO X. A study of hazards of soil heavy metals Cu stress on plants[J]. Open Journal of Nature Science, 2014, 2: 18-23.
[1]	HU M M, WANG Q, YANG Y Z. Accumulation, distribution and excretion of heavy metals in insects[J]. Chinese Agricultural Science Bulletin, 2012, 28(18):213-217.
[2]	DING P, ZHUANG P, LI Z, et al. Accumulation and detoxification of cadmium by larvae of Prodenia litura (Lepidoptera: Noctuidae) feeding on Cd-enriched amaranth leaves[J]. Chemosphere, 2013, 91(1): 28-34.
[2]	YANG S Y, HUANG Y J, ZHANG M, et al. Ecophysiological effects of heavy metals on insects[J]. Acta Entomologica Sinica, 2015,58(4):427-436.
[3]	WU Q X, XIA Q. Effect of heavy metal on antioxidant enzymes in insects[J].Journal of Environmental Entomology,2014,36(2): 247-251.
[3]	LANNONE M F, GROPPA M D, BENAVIDES M P. Cadmium induces different biochemical responses in wild type and catalase-deficient tobacco plants[J]. Environmental and Experimental Botany, 2015, 109: 201-211.
[4]	WANG H, WU G X, YE G Y, et al. Accumulation of cuprum and cadmium and their effects on the antioxidant enzymes in Boettcherisca peregrine exposed to cuprum and cadmium[J]. Journal of Zhejiang University(Agric Life Sci),2006,32(1):77-81.
[4]	LI S H, ZHANG G J, GAO W J, et al. Plant growth, development and change in GSH level in safflower (Carthamus tinctorius L.) exposed to copper and lead[J]. Archives of Biological Sciences, 2015, 1: 6.
[5]	GAO H H. Defense mechanism of Sitobion avenae (Hemiptera: Aphididae) under the long-term exposure of cadmium and zinc[D]. Yangling: Northwest A F University, 2013.
[5]	胡蒙蒙, 王青, 杨益众. 重金属在昆虫体内的累积、分布与排泄[J]. 中国农学通报, 2012, 28(18): 213-217.
[6]	NIU Y Q, ZHENG J F, WANG H L. Progress in effect of lead on anti-oxidative damage enzyme of aquatic animal[J]. Environmental Science and Technology,2010(2):74-78.
[6]	杨世勇, 黄永杰, 张敏, 等. 重金属对昆虫的生态生理效应[J].昆虫学报, 2015, 58(4): 427-436.
[7]	LIU W G, ZHANG X D, HUANG L L, et al. Research progress on physiologic and ecologic characteristics of poplar[J].World Forestry Research, 2010,23(1):50-55.
[7]	吴启仙, 夏嫱. 重金属对昆虫抗氧化酶影响研究进展[J]. 环境昆虫学报, 2014, 36(2): 247-251.
[8]	王慧, 吴国星, 叶恭银, 等. 铜和镉在棕尾别麻蝇体内的累积及其对三种抗氧化酶活性的影响[J]. 浙江大学学报(农业与生命科学版), 2006, 32(1): 77-81.
[8]	Environmental quality standard for soils: GB15618[S]. Beijing: China Standard Press,1995.
[9]	MENG Z H, ZHOU J, ZHENG Y F. Ecological risk assessment of heavy metal elements in urban soil of Harbin[J]. Research of Soil and Water Conservation,2009,16(2):153-159.
[9]	高欢欢. 重金属Cd和Zn长期胁迫下麦长管蚜Sitobion avenae防御机制的研究[D]. 杨凌:西北农林科技大学, 2013.
[10]	LU Y F, YAN J X, LI S W, et al. Effects of the Larix gmelinii grown under different light intensities on the development and defensive enzyme activities of Lymantria dispar larvae[J]. Acta Ecologica Sinica, 2013,33(22):7125-7131.
[10]	MIRACˇU2IAC＇U2 D, BLAGOJEVIAC＇U2 D, PERIAC＇U2-MATARUGA V, et al. Cadmium effects on the fitness-related traitsand antioxidative defense of Lymantria dispar L. larvae[J].Environ Sci Pollut Res, 2013, 20: 209-218.
[11]	牛亚青, 郑济芳, 王晗莉. 重金属铅对水生动物抗氧化损伤酶的影响研究进展[J]. 环境科技, 2010(2):74-78.
[11]	KONG X P, LI S W, CUI W C, et al. Effects of different light intensities on the development and defensive enzyme activities of Lymantria dispar[J]. Journal of Northeast Forestry University,2013,41(7):104-107.
[12]	刘文国, 张旭东, 黄玲玲, 等. 我国杨树生理生态研究进展[J]. 世界林业研究, 2010, 23(1):50-55.
[12]	YAN J M, LIAO Y Z, YAN S C, et al. Effects of methoxyfenozide on the activities of detoxifying enzymes and protective enzymes in Lymantria dispar (Lepidoptera: Lymantriidae)[J]. Journal of Northeast Forestry University,2010,38(11):112-114.
[13]	ROBERT L H, MELODY A K, DANIEL L R. Classification, genetic variation and pathogenicity of Lymantria dispar nucleopolyhedrovirus isolates from Asia, Europe, and North America[J]. Journal of Invertebrate Pathology, 2014, 116:27-35.
[13]	FENG C F, YAN S C, LU Y F, et al. Effects of induced resistance of Larix gmelinii on the activities of detoxifying enzymes in Lymantria dispar[J]. Scientia Silvae Sinicae,2011,47(8):102-107.
[14]	中华人民共和国国家标准土壤环境质量标准:GB15618[S]. 北京: 中国标准出版社, 1995.
[14]	LI H, YAN S C, CAO C W. Effects of furan tebufenozide on activities of detoxifying enzymes of Lymantria dispa[J].Journal of Northeast Forestry University,2011,39(9):97-100.
[15]	孟昭虹, 周嘉, 郑元福. 哈尔滨市城市土壤重金属生态风险评价[J]. 水土保持研究, 2009, 16(2): 153-159.
[15]	WANG Y, LI L J, ZHANG Y P, et al. Accumulation of chromium in Oxya chinensis and its effects on the antioxidant system[J]. Journal of Agro-Environment Science,2010,29(12):2281-2286.
[16]	LIU Y M, YANG H M, ZHANG Y P, et al. Acute effects of Cd2+ and Cr6+ on detoxification enzymes and polyphenol oxidase in Oxya chinensis[J]. Journal of Agro-Environment Science, 2013,32(7):1321-1327.
[16]	鲁艺芳, 严俊鑫, 李霜雯, 等. 不同光照强度下兴安落叶松对舞毒蛾幼虫生长发育及防御酶的影响[J]. 生态学报, 2013, 33(22): 7125-7131.
[17]	孔祥鹏, 李霜雯, 崔伟婵, 等. 不同光照强度对舞毒蛾生长发育及防御酶活性的影响[J]. 东北林业大学学报, 2013, 41(7): 104-107.
[17]	HAO R Z, YU Y, WU J Y, et al. Research progress on mechanisms of metal resistance in trees[J]. Chinese Agricultural Science Bulletin, 2012,28(10):6-12.
[18]	ZHANG K, XU B, MENG Z J, et al. Effect of copper and cadmium on defensive protein activity in poplar leaves[J]. Journal of Northeast Forestry University,2014,42(11):43-46.
[18]	鄢杰明, 廖月枝, 严善春, 等. 甲氧虫酰肼对舞毒蛾解毒酶和保护酶活性的影响[J]. 东北林业大学学报, 2010, 38(11): 112-114.
[19]	冯春富, 严善春, 鲁艺芳, 等. 兴安落叶松诱导抗性对舞毒蛾幼虫解毒酶活性的影响[J]. 林业科学, 2011, 47(8): 102-107.
[20]	李慧, 严善春, 曹传旺. 呋喃虫酰肼对舞毒蛾解毒酶活力的影响[J]. 东北林业大学学报, 2011, 39(9): 97-100.
[21]	BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical biochemistry, 1976, 72(1): 248-254.
[22]	王跃, 李丽君, 张育平, 等. 铬在中华稻蝗(Oxya chinensis)体内的累积及对抗氧化系统的影响[J]. 农业环境科学学报, 2010, 29(12): 2281-2286.
[23]	刘耀明, 杨慧敏, 张育平, 等. 镉和铬急性染毒对中华稻蝗解毒酶及多酚氧化酶的影响[J]. 农业环境科学学报, 2013, 32(7): 1321-1327.
[24]	WU G Q, YI Y H. Effects of dietary heavy metals on the immune and antioxidant systems of Galleria mellonella larvae[J]. Comparative Biochemistry and Physiology, 2015,167:131-139.
[25]	郝瑞芝, 余洋, 武佳叶, 等. 树木对重金属的抗性机理研究进展[J]. 中国农学通报, 2012, 28(10): 6-12.
[26]	张凯, 徐波, 孟昭君, 等. 铜、镉胁迫对杨树叶片中防御蛋白活性的影响[J]. 东北林业大学学报, 2014, 42(11):43-46.

Cited By

Cited by

Periodical cited type(5)

1.	王尚晓，张晓东，张明，牛晓楠，周墨，唐志敏，张洁，宗乐丽，徐帅. 基于RF和EBKRP算法的新安江流域有效土壤厚度反演. 水土保持通报. 2025(01): 168-177 .
2.	舒兴华，秦中权，王酸，高黎明，黄红梅. 基于DEM的坡位图生成方法. 安徽林业科技. 2024(03): 52-55 .
3.	陈永富，陈巧，刘华. 林地小班边界及地形因子计算机自动提取研究及应用进展. 林业资源管理. 2021(04): 166-172 .
4.	王媛媛. 基于DEM的陕北黄土高原地形因子的提取与分析. 西部大开发(土地开发工程研究). 2019(12): 28-36 .
5.	唐玉芝，邵全琴. 乌江上游森林生态系统水源涵养数据集. 全球变化数据学报(中英文). 2018(04): 433-441+551-559 .

Other cited types(6)

Get Citation

PDF

XML

Article views (1639) PDF downloads (22) Cited by(11)

Turn off MathJax

Article Contents

Abstract

References

Effects of copper and cadmium stress on the activities of detoxifying metabolic enzymes in Lymantria dispar

Abstract

References

Cited by

Periodical cited type(5)

Other cited types(6)

Catalog

Export File

Citation

Format

Content