Combined ABTS radical scavenging activity of Pinus koraiensis polyphenols with fungus polysaccharides.

We compared the ABTS radical scavenging activity of Pinus korsiensis polyphenols (PKP) alone or combined with Auricularia auricular polysaccharides (AAP) and Ganoderma lucidum spore polysaccharides (GLP). ABTS radical scavenging experiments and Chou-Talalay combination index (CI) method were used to evaluate the scavenging effects of PKP with AAP and GLP individually as well as in combination, and combination index (CI), isobologram and dose-reduction index (DRI) were worked out subsequently. It showed that IC50 values of PKP, AAP and GLP were 72.01 μg/mL, 2.49 mg/mL and 0.93 mg/mL, respectively, while IC50 values of the combination (mass ratio 1∶3) were 185.20 and 237.83 μg/mL, respectively. CI analysis indicated that the clearance rate was 10%-97%, and CI of PKP with AAP was less than 1. Dose of PKP with AAP was reduced based on isobologram. DRI analysis demonstrated that the clearance rate was 5%-97%, and DRI of PKP with AAP was greater than 1. The results of PKP with GLP were inconsistent. Our study suggests that the combination of PKP and AAP has significantly improved the ABTS radical scavenging activity, compared with using them individually.