Combined ABTS radical scavenging activity of Pinus koraiensis polyphenols with fungus polysaccharides.

ZHANG Nai-xun; YIN Hong-li; ZHAO Xin; LIU Ran; YU Mei-hui; WANG Zhen-yu

doi:10.13332/j.1000-1522.20150527

Journal of Beijing Forestry University > 2016 > 38(10): 104-111. > DOI: 10.13332/j.1000-1522.20150527

ZHANG Nai-xun, YIN Hong-li, ZHAO Xin, LIU Ran, YU Mei-hui, WANG Zhen-yu. Combined ABTS radical scavenging activity of Pinus koraiensis polyphenols with fungus polysaccharides.[J]. Journal of Beijing Forestry University, 2016, 38(10): 104-111. DOI: 10.13332/j.1000-1522.20150527

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Combined ABTS radical scavenging activity of Pinus koraiensis polyphenols with fungus polysaccharides.

1.
1 College of Forestry, Northeast Forestry University, Harbin, Heilongjiang, 150040, P. R. China;
2.
2 Institute of Food Science and Technology CAAS/Institute of Staple Food Processing Technology, Harbin, Heilongjiang, 151900, P. R. China;
3.
3 Agricultural College, Liaocheng University, Liaocheng, Shandong, 252000, P. R. China;
4.
4 Department of Food Science and Engineering, Harbin Institute of Technology, Harbin, Heilongjiang, 150090, P. R. China.

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Received Date: December 29, 2015
Published Date: October 28, 2016

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Abstract

Abstract

We compared the ABTS radical scavenging activity of Pinus korsiensis polyphenols (PKP) alone or combined with Auricularia auricular polysaccharides (AAP) and Ganoderma lucidum spore polysaccharides (GLP). ABTS radical scavenging experiments and Chou-Talalay combination index (CI) method were used to evaluate the scavenging effects of PKP with AAP and GLP individually as well as in combination, and combination index (CI), isobologram and dose-reduction index (DRI) were worked out subsequently. It showed that IC50 values of PKP, AAP and GLP were 72.01 μg/mL, 2.49 mg/mL and 0.93 mg/mL, respectively, while IC50 values of the combination (mass ratio 1∶3) were 185.20 and 237.83 μg/mL, respectively. CI analysis indicated that the clearance rate was 10%-97%, and CI of PKP with AAP was less than 1. Dose of PKP with AAP was reduced based on isobologram. DRI analysis demonstrated that the clearance rate was 5%-97%, and DRI of PKP with AAP was greater than 1. The results of PKP with GLP were inconsistent. Our study suggests that the combination of PKP and AAP has significantly improved the ABTS radical scavenging activity, compared with using them individually.
- fungus polysaccharides,
- Pinus koraiensis polyphenols,
- combination,
- antioxidant activity

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References

[1]	ZHANG N. Study on preparation and antioxidant activities of fractionation polysaccharide by alcohol in Auricularia auricula[D]. Harbin: Northeast Forestry University, 2011.
[1]	LECUMBERRI E, DUPERTUIS Y M, MIRALBELL R, et al. Green tea polyphenol epigallocatechin-3-gallate (EGCG) as adjuvant in cancer therapy[J]. Clinical Nutrition, 2013, 32(6): 894-903.
[2]	FAN L S, GONG C R, ZHANG S H.Study on radio-protective effects of the polysaccharide from Auricular auricula in mice[J]. Acta Nutrimenta Sinica, 2005, 27(6): 525-526.
[2]	HUANG W W, TANG J S, LIN C F, et al. Pycnogenol induces differentiation and apoptosis in human promyeloid leukemia HL-60 cells[J]. Leukemia Research, 2005, 29(6): 685-692.
[3]	PENG Y J, LEE C H, WANG C C, et al. Pycnogenol attenuates the inflammatory and nitrosative stress on joint inflammation induced by urate crystals[J]. Free Radical Biology and Medicine, 2012, 52(4): 765-774.
[3]	JIAO Y L, WEN S N, DU B, et al. Effect of twin-screw extrusion conditions on polysaccharide extraction from Ganoderma lucidum spore powder[J]. Food Science, 2011, 32(16): 67-70.
[4]	KIM Y J, KIM Y A, YOKOZAWA T. Pycnogenol modulates apoptosis by suppressing oxidative stress and inflammation in high glucose-treated renal tubular cells[J]. Food and Chemical Toxicology, 2011, 49(9): 2196-2201.
[4]	WANG J Q, NIE S P, YU Q, et al. Immune modulation and antioxidation activity of polysaccharides from Ganoderma atrum in immunosuppressed mice[J]. Food Science, 2012, 33(23): 274-277.
[5]	GAO H L, LONG Y Z, JIANG X Q, et al. Beneficial effects of Yerba Mate tea (Ilex paraguariensis) on hyperlipidemia in high-fat-fed hamsters[J]. Experimental Gerontology, 2013, 48(6): 572-578.
[5]	The Ministry of Agriculture of the Peoples Republic of China. Determination of crude mushroom polysaccharides:NY/T1676—2008[S]. Beijing: China Agriculture Press, 2008.
[6]	YANG Y S, AHN T H, LEE J C, et al. Protective effects of pycnogenol on carbon tetrachloride-induced hepatotoxicity in Sprague-Dawley rats[J]. Food and Chemical Toxicology, 2008, 46(1): 380-387.
[6]	The State Administration of Quality Supervision, Inspection and Quarantine of the Peoples Republic of China, China National Standardization Management Committee. Determination of total polyphenols and catechins content in tea: GB/T8313—2008[S]. Beijing: China Standard Press, 2008.
[7]	WANG Y F, LI L, XIE Z Z, et al.Study on antioxidant activity and synergistic effect of tea polyphenols and Panax quinquefolium[J]. China Foreign Medical Treatment, 2008, 27(29): 68.
[7]	张宁. 黑木耳分级多糖制备及抗氧化研究[D]. 哈尔滨: 东北林业大学, 2011.
[8]	BAI H N, WANG Z Y, LI H, et al. Effect of five berry polyphenols and Auricularia auricular polysaccharides combination on radiation protection[J]. Science and Technology of Food Industry, 2013, 34(16): 117-120,124.
[8]	樊黎生, 龚晨睿, 张声华. 黑木耳多糖抗辐射效应的动物实验[J]. 营养学报, 2005, 27(6): 525-526.
[9]	MA J W, QIAO Z Y, XIANG X. Optimisation of extraction procedure for black fungus polysaccharides and effect of the polysaccharides on blood lipid and myocardium antioxidant enzymes activities[J]. Carbohydrate Polymers, 2011, 8: 1061-1068.
[9]	BAI H N, WANG Z Y, ZHANG H, et al. Study on synergistic effect of polyphenols and Auricularia auricular polysaccharides combination on antioxidant activity[J]. Science and Technology of Food Industry, 2013, 34(22): 124-127,134.
[10]	SHI M, YANG Y N, HU X S, et al. Effect of ultrasonic extraction conditions on antioxidative and immunomodulatory activities of a Ganoderma lucidum polysaccharide originated from fermented soybean curd residue[J]. Food Chemistry, 2014, 155: 50-56.
[11]	SUN X B, ZHAO C, PAN W, et al. Carboxylate groups play a major role in antitumor activity of Ganoderma applanatum polysaccharide[J]. Carbohydrate Polymers, 2015, 123: 283-287.
[12]	ZHU K X, NIE S P, LI C, et al. A newly identified polysaccharide from Ganoderma atrum attenuates hyperglycemia and hyperlipidemia[[J]. International Journal of Biological Macromolecules, 2013, 57: 142-150.
[13]	焦艳丽, 温升南, 杜冰, 等. 挤压处理灵芝孢子粉提取灵芝多糖[J]. 食品科学, 2011, 32(16): 67-70.
[14]	LI X L, ZHOU A G, LI X M. Inhibition of Lycium barbarum polysaccharides and Ganoderma lucidum polysaccharides against oxidative injury induced by γ-irradiation in rat liver mitochondria[J]. Carbohydrate Polymers, 2007, 69(1): 172-178.
[15]	JIA J, ZHANG X, HU Y S, et al. Evaluation of in vivo antioxidant activities of Ganoderma lucidum polysaccharides in STZ-diabetic rats[J]. Food Chemistry, 2009, 115(1): 32-36.
[16]	王君巧, 聂少平, 余强, 等. 黑灵芝多糖对免疫抑制小鼠的免疫调节和抗氧化作用[J]. 食品科学, 2012, 33(23): 274-277.
[17]	FIGUERO E, SOORY M, CERERO R, et al. Oxidant/antioxidant interactions of nicotine, coenzyme Q10, pycnogenol and phytoestrogens in oral periosteal fibroblasts and MG63 osteoblasts[J]. Steroids, 2006, 71(12): 1062-1072.
[18]	中华人民共和国农业部. 食用菌中粗多糖含量的测定:NY/T1676—2008[S]. 北京: 中国农业出版社, 2008.
[19]	中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. 茶叶中茶多酚和儿茶素类含量的检测方法:GB/T8313—2008[S]. 北京: 中国标准出版社, 2008.
[20]	CHOU T C. Drug combination studies and their synergy quantification using the Chou-Talalay Method [J]. Cancer Res, 2010, 70(2): 440-446.
[21]	BOATH A S, STEWART D, MCDOUGALL G J. Berry components inhibit α-glucosidase in vitro: synergies between acarbose and polyphenols from black currant and rowanberry[J]. Food Chemistry, 2012, 135(3): 929-936.
[22]	王岳飞, 李磊, 谢争珍, 等. 茶多酚西洋参抗氧化活性比较及协同增效作用研究[J]. 中外医疗, 2008, 27(29): 68.
[23]	白海娜, 王振宇, 李辉, 等. 五种浆果多酚与黑木耳多糖复合物的辐射防护作用[J]. 食品工业科技, 2013, 34(16): 117-120,124.
[24]	白海娜, 王振宇, 张华, 等. 多酚类化合物与黑木耳多糖协同抗氧化作用研究[J]. 食品工业科技, 2013, 34(22): 124-127,134.
[25]	AMORATI R, FERRONI F, PEDULLI G F, et al. Modeling the co-antioxidant behavior of monofunctional phenols: applications to some relevant compounds[J]. The Journal of Organic Chemistry, 2003, 58: 9654-9658.
[26]	ROBERTS W G, GORDON M H. Determination of the total antioxidant activity of fruits and vegetables by a liposome assay[J]. Journal of Agricultural and Food Chemistry, 2003, 51: 1486-1493.
[27]	JORGENSEN L V, MADSEN H L, THOMSEN M K, et al. Regeneration of phenolic antioxidants from phenoxyl radicals: an ESR and electrochemical study of antioxidant hierarchy[J]. Free Radical Research, 1999, 30(3): 207-220.
[28]	FERREIRA R D Q, GRECO S J, DELARMELINA M, et al. Electrochemical quantification of the structure/antioxidant activity relationship of flavonoids[J]. Electrochimica Acta, 2015, 163 (5): 161-166.
[29]	ZHANG H N, MA H L, LIU W, et al. Ultrasound enhanced production and antioxidant activity of polysaccharides from mycelial fermentation of Phellinus igniarus[J]. Carbohydrate Polymers, 2014, 113: 380-387.
[30]	THAMBIRAJ S R, PHILLIPS M, KOYYALAMMUDI S R, et al. Antioxidant activities and characterisation of polysaccharides isolated from the seeds of Lupinus angustifolius[J]. Industrial Crops and Products, 2015, 74: 950-956.

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