EST-SSR analysis of genetic diversity of Robinia pseudoacacia clones in Jixian County, Shanxi Province of northern China

Objective This paper aims to understand the number of alleles of simple sequence repeats detected by EST-SSR primers, and to analyze the polymorphisms of germplasm of Robinia pseudoacacia clones in Ji xian County of Shanxi Province,northern China and the genetic relationship between germplasms, and provide reference for primer utilization and genetic management of Robinia pseudoacacia germplasm.

Method 45 pairs of EST-SSR primers developed by our laboratory were used for PCR amplification, and the products were detected by capillary electrophoresis, the genetic parameters of population were evaluated by Popgene 1.32 software, and cluster analysis was performed by NTsys 2.10e.

Result Except for primer RP-24, 45 pairs of EST-SSR primers showed good polymorphism. A total of 298 allelic repeat sequences were detected in 44 pairs of primers, and 2 to 15 were detected in each pair, with an average of 6.77. The observed heterozygosity (H_o) of the population ranged from 0.022 7 to 0.842 1, with an average of 0.412 6. The expected heterozygosity (H_e) varies from 0.088 7 to 0.822 3, averaging 0.484 0. Primer polymorphism information (PIC) ranged from 0.085 2 to 0.796 6, with an average of 0.444 4.

Conclusion Robinia pseudoacacia clonal germplasm in this base has high genetic diversity. It can be seen from the clustering results that 96 genetic clones of the hedgehog clones can be clustered into five classes when the genetic distance is 0.81. Cluster maps showed the genetic relationship among the clones of Robinia pseudoacacia at the molecular level, and the results of the study have positive reference value for the use of these primers for germplasm evaluation, resource management and assisted breeding of Robinia pseudoacacia.