Comparative analysis of polar phase metabolite in wood forming tissue of normal wood and reaction wood in Pinus koraiensis.

Many metabolic pathways participate in xylem formation (wood formation) progress of secondary growth in tree. Metabolite analysis has become an important tool for function identification of genes and molecule mechanism exploration of biological development. To identify main metabolites of wood forming tissue and their roles in this progress, and then give insight into bioprocess of wood formation, polar metabolites were extracted from wood forming tissue of compression wood, opposite wood and normal used methanol/chloroform organic solvent systems. Chemical compound and relative abundance were analyzed and identified by gas chromatograph/mass spectrometry (GCMS). Thirty\|one metabolites were identified in this research. Relative abundance of major metabolites from compression wood was lower than or close to normal wood, while sugars decreased obviously. Relative abundance of major metabolites from opposite wood was close to or higher than normal wood, Ncompounds and lipids increased distinctly. Relative abundance of major metabolites from compression wood was lower than opposite wood. It could reflect metabolic network way of wood development on integral level by metabonomics, also provide evidence to identify key regulate factor of wood formation.