• Scopus
  • Chinese Science Citation Database (CSCD)
  • A Guide to the Core Journal of China
  • CSTPCD
  • F5000 Frontrunner
  • RCCSE
Advanced search
ZHENG Dong-chao, XIA Xin-li, YIN Wei-lun. Auxin promotes nitrate uptake by upregulating AtNRT1.1 gene transcript level in Arobidopsis thaliana[J]. Journal of Beijing Forestry University, 2013, 35(2): 80-85.
Citation: ZHENG Dong-chao, XIA Xin-li, YIN Wei-lun. Auxin promotes nitrate uptake by upregulating AtNRT1.1 gene transcript level in Arobidopsis thaliana[J]. Journal of Beijing Forestry University, 2013, 35(2): 80-85.

Auxin promotes nitrate uptake by upregulating AtNRT1.1 gene transcript level in Arobidopsis thaliana

More Information
  • Received Date: December 31, 1899
  • Revised Date: December 31, 1899
  • Published Date: March 29, 2013
  • In order to study the mechanisms of the effects of auxin signaling pathway on nitrate uptake, scanning ionselective electrode technique(SIET) and quantitative realtime PCR technique were used to determine net plasma membrane NO-3 fluxes and the expression of nitrate transporter AtNRT1.1 in primary roots of Arabidopsis thaliana wild type Col-0, auxin overproducing mutant yuc1-D, and auxin insensitive mutant axr1-12. Moreover, we examined net plasma membrane NO-3 fluxes and the expression of AtNRT1.1 in primary roots of Col-0 and dual-affinity nitrate transporter mutant nrt1.1 seedlings under the normal condition(CK), the treatment with exogenous IAA or the treatment with the auxin efflux inhibitor 2.3.5triidobenzoid acid (TIBA). The nitrate uptake and AtNRT1.1 gene transcript level in yuc1-D seedlings were remarkably enhanced, whereas those in axr1-12 seedlings declined compared with Col-0 seedlings. The exogenous IAA treatment enhanced nitrate uptake and AtNRT1.1 gene transcript level in Col-0 seedlings, while the TIBA treatment had an opposite effect. Furthermore, the differences of nitrate uptake and AtNRT1.1 gene transcript level in nrt1.1 seedlings among CK, IAA and TIBA treatments were insignificant compared with Col-0 seedlings, revealing the important role that AtNRT1.1 gene played in nitrate uptake pathway response to auxin.
  • Related Articles

    [1]Li Lijing, Zhang Zhiwei, Xue Yun, Zhang Jiahang, Han Liebao, Xu Lixin. Effects of low temperature stress on chlorophyll metabolism of Zoysia japonica[J]. Journal of Beijing Forestry University, 2022, 44(2): 91-99. DOI: 10.12171/j.1000-1522.20200400
    [2]Chen Shenggang, Pang Danbo, Chen Lin, Bian Yingying, Wan Hongyun, He Wenqiang, Cao Menghao. Response of growth and metabolism of Artemisia scoparia to precipitation change in desert steppe[J]. Journal of Beijing Forestry University, 2022, 44(2): 65-74. DOI: 10.12171/j.1000-1522.20210413
    [3]Li Yang, Hao Xin, Li Mingrui, Diao Jian, Ma Ling. Functional analysis of P-glycoprotein in drug metabolism of Bursaphelenchus xylophilus[J]. Journal of Beijing Forestry University, 2021, 43(11): 71-78. DOI: 10.12171/j.1000-1522.20200387
    [4]Wu Shuai, Jiang Dun, Ma Qinghui, Tan Mingtao, Zhao Jiaqi, Liu Xiaoxia, Meng Zhaojun, Yan Shanchun. Effects of arbuscular mycorrhizal fungi on metabolism and chemical defense of Populus alba × P. berolinensis leaves[J]. Journal of Beijing Forestry University, 2021, 43(5): 86-92. DOI: 10.12171/j.1000-1522.20200172
    [5]ZHANG Kai, ZHOU Yan-tao, WANG Xi-wei, JIANG Hong, WANG Jia-bing, ZHANG Wen-yi, YAN Shan-chun. Effects of copper and cadmium stress on the activities of detoxifying metabolic enzymes in Lymantria dispar[J]. Journal of Beijing Forestry University, 2016, 38(2): 61-67. DOI: 10.13332/j.1000-1522.20150293
    [6]GAO Qiong, NIU Shi-hui, LI Wei, CHEN Xiao-yang. Regulation of low temperature stress on gibberellin metabolism[J]. Journal of Beijing Forestry University, 2014, 36(6): 135-141. DOI: 10.13332/j.cnki.jbfu.2014.06.025
    [7]YIN Da-chuan, DENG Xun, ZHENG Yu, SONG Rui-qing. Effects of salt-alkali stress on growth and nutritional metabolism of Trichoderma harzianum T28[J]. Journal of Beijing Forestry University, 2014, 36(5): 109-116. DOI: 10.13332/j.cnki.jbfu.2014.05.018
    [8]CHEN Li-pei, YANG Bo, SHEN Yong-bao. Endogenous hormone content of endosperm of Pinus tabuliformis seeds during initial germinating stage[J]. Journal of Beijing Forestry University, 2012, 34(6): 30-33.
    [9]KONG Ying, SUN Ming, PAN Hu-tang, ZHANG Qi-xiang. Advances in metabolism and regulation of floral scent.[J]. Journal of Beijing Forestry University, 2012, 34(2): 146-154.
    [10]HE Yao-qing, ZHENG Cai-xia. Relationship between metabolism in developmental ovule and female-sterile in Pinus tabulaeformis Carr[J]. Journal of Beijing Forestry University, 2007, 29(5): 88-93. DOI: 10.13332/j.1000-1522.2007.05.016

Catalog

    Article views (2165) PDF downloads (100) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return