Inhibition effects of biocontrol bacteria strains on the pathogen of <i>Camellia oleifera</i> anthracnose

Zhang Ping; Li Chaoyang; Zhao Qingquan; Wang Lihai; Ma Ling

doi:10.12171/j.1000-1522.20190409

Journal of Beijing Forestry University > 2020 > 42(10): 107-116. > DOI: 10.12171/j.1000-1522.20190409

Zhang Ping, Li Chaoyang, Zhao Qingquan, Wang Lihai, Ma Ling. Inhibition effects of biocontrol bacteria strains on the pathogen of Camellia oleifera anthracnose[J]. Journal of Beijing Forestry University, 2020, 42(10): 107-116. DOI: 10.12171/j.1000-1522.20190409

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Inhibition effects of biocontrol bacteria strains on the pathogen of Camellia oleifera anthracnose

1.
Heilongjiang Forest Protection Technology Innovation Center, Northeast ForestryUniversity, Harbin 150040, Heilongjiang, China
2.
College of Engineering Technology, Northeast Forestry University, Harbin 150040, Heilongjiang, China

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Received Date: October 13, 2019
Revised Date: April 02, 2020
Available Online: September 23, 2020
Published Date: October 24, 2020

Graphical Abstract

Abstract

Abstract

Objective Endophytic bacteria with antagonistic action on oil tea anthracnose pathogen Colletotrichum fructicola were isolated from the fruit of Physali alkekengi, followed by the study on the antibacterial mechanism of the strain on the pathogen of Camellia oleifera anthracnose. It is expected to provide a theoretical basis for the field control of Camellia oleifera anthracnose.
Method In this study, fourteen strains of bacteria were isolated from fruits by tissue separation isolation, and then three strains of bacteria with significant inhibitory effects on the pathogen of Camellia oleifera anthracnose were obtained with plate-screening method, including the strains of DLSB-1, DLSB-4 and DLSB-13. Subsequently, combining strain morphology, physiological and biochemical characteristics and 16S rDNA sequence alignment analysis, the strain was identified. Furthermore, based on the above test, whether the biocontrol strain can produce protease, cellulase and β-1,3-glucanase was determined, along with the observation of the mycelial growth of pathogenic hyphae fungus by antagonistic bacteria sterile fermentative liquid with microscope, and the indoor control effect of Camellia oleifera seedlings was measured indoors.
Result It was found that all three strains of Bacillus sterile fermentative liquid could inhibit the normal growth of the pathogenic hyphae of Camellia oleifera anthracnose, thus making it tip-expanding, deformity and distorted. Besides, protease and cellulase could be produced, while β-1,3-glucanase could not be produced; when compared 5 concentrations (0.6 × 10⁸, 1.0 × 10⁸, 1.4 × 10⁸, 1.8 × 10⁸, 2.2 × 10⁸ cfu/mL) of sterile fermentative liquid with control, it was found that the antibacterial effect on the pathogen of Camellia oleifera anthracnose was significant (P < 0.05), with the highest level of 81.14%. Moreover, the control effects of 5 concentrations of sterile fermentative liquid of strain DLSB-4 on potted Camellia oleifera seedlings were 40.2%, 43.9%, 57.7%, 68.7% and 71.1%, respectively. All these show that there is a positive correlation between good control effects as well as the concentration of sterile fermentative liquid and the control effect.
Conclusion The three strains of biocontrol bacteria could produce antagonistic enzymes which inhibiting the growth of pathogenic bacteria hyphae of Colletotrichum fructicola, thus reducing the incidence of Camellia oleifera anthracnose.
- Bacillus,
- Colletotrichum fructicola,
- inhibition effect,
- biocontrol,
- endophytic bacteria

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References (30)

References

[1]	张凌云, 王小艺, 曹一博. 油茶果实糖含量及代谢相关酶活性与油脂积累关系分析[J]. 北京林业大学学报, 2013, 35(4):55−60. Zhang L Y, Wang X Y, Cao Y B. Soluble sugar content and key enzyme activity and the relationship between sugar metabolism and lipid accumulation in developing fruit of Camellia oleifera[J]. Journal of Beijing Forestry University, 2013, 35(4): 55−60.
[2]	杨华, 赵丹阳, 秦长生, 等. 不同油茶种和品种对炭疽病的抗性观察[J]. 植物保护, 2015, 41(2):195−199. Yang H, Zhao D Y, Qin C S, et al. Resistance of different species and cultivars of oil-tea Camelliato colletotrichum gloeosporioides[J]. Plant Protection, 2015, 41(2): 195−199.
[3]	信珊珊, 祁高富, 朱发银, 等. 1株解淀粉芽孢杆菌发酵条件的优化及其对油茶炭疽病的防效[J]. 华中农业大学学报, 2011, 30(4):411−415. Xin S S, Qi G F, Zhu F Y, et al. Optimization of fermentation condition for Bacillus amyloliquefaciens WH1 and its biological control[J]. Journal of Huazhong Agricultural University, 2011, 30(4): 411−415.
[4]	李石磊, 刘君昂, 赵莹, 等. 6种杀菌剂对油茶主要病害的室内毒力测定[J]. 中南林业科技大学学报, 2013, 33(9):60−62. Li S L, Liu J A, Zhao Y, et al. Indoor toxicity determination of six fungicides on main diseases of Camellia oleifera[J]. Central South University of Forestry & Technology, 2013, 33(9): 60−62.
[5]	Jia Y X, Yang M X, Wang H, et al. First report of anthracnose on Stylosanthes guianensis caused by Colletotrichum karstii in China[J]. Plant Disease, 2017, 101(4): 630−631.
[6]	Wang J, Ai C X, Yu X M, et al. First report of Colletotrichum karstii causing anthracnose on persimmon leaves in China[J]. Plant Disease, 2016, 100(2): 532.
[7]	李凤, 刘会涛, 田莎, 等. 复配芽孢杆菌对撑绿杂交竹枯萎病的生物防治效果研究[J]. 北京林业大学学报, 2018, 40(12):76−84. Li F, Liu H T, Tian S, et al. Biological control effect of compounded Bacillus on wilt disease of Bambusa pervariabilis ×Dendrocalamopsis daii[J]. Journal of Beijing Forestry University, 2018, 40(12): 76−84.
[8]	Selim S, Sanssene J, Rossard S, et al. Systemic induction of the defensin and phytoalexin Pisatin pathways in pea (Pisum sativum) against Aphanomyces euteiches by acetylated and nonacetylated oligogalacturonides[J/OL]. Molecules, 2017, 22(6): 1017 [2019−10−11]. https://www.mdpi.com/1420-3049/22/6/1017.
[9]	Shifa H, Gopalakrishnan C, Velazhahan R. Management of late leaf spot (Phaeoisariopsis personata) and root rot (Macrophomina phaseolina) diseases of groundnut (Arachis hypogaea L.) with plant growth-promoting rhizobacteria, systemic acquired resistance inducers and plant extractsnd plant extracts[J]. Phytoparasitica, 2018, 46(1): 19−30. doi: 10.1007/s12600-018-0644-z
[10]	Chinnaswamy A, Coba de la Peña T, Stoll A, et al. A nodule endophytic Bacillus megaterium strain isolated from Medicago polymorpha enhances growth, promotes nodulation by Ensifer medicae and alleviates salt stress in alfalfa plants[J]. Annals of Applied Biology, 2018, 172(3): 295−308. doi: 10.1111/aab.12420
[11]	Hallmann J, Quadt-Hallmann A, Mahaffee W F, et al. Bacterial endophytes in agricultural crops[J]. Canadian Journal of Microbiology, 1997, 43(10): 895−914. doi: 10.1139/m97-131
[12]	Xu W, Ren H, Ou T, et al. Genomic and functional characterization of the endophytic Bacillus subtilis 7PJ-16 strain, a potential biocontrol agent of mulberry fruit sclerotiniose[J]. Microbial Ecology, 2018, 14(4): 15488−15504.
[13]	魏蜜, 张伟, 管维轩, 等. 1株油茶真菌病害拮抗菌株的筛选鉴定及其拮抗作用[J]. 江苏农业科学, 2017, 45(18):100−104. Wei M, Zhang J, Guan W X, et al. Screening, identification and antagonism of an antagonistic strain of oil-tea fungal disease[J]. Jiangsu Agricultural Sciences, 2017, 45(18): 100−104.
[14]	高月庭, 周国英, 何小勇, 等. 拮抗细菌Y13对油茶炭疽病防治试验[J]. 浙江林业科技, 2014, 34(2):14−17. Gao Y T, Zhou G Y, He X Y, et al. Experiment on prevention and control of anthracnose in adult Camellia oleifera stand by Y13[J]. Journal of Zhejiang Forestry Science and Technology, 2014, 34(2): 14−17.
[15]	王万能, 全学军, 肖崇刚. 植物诱导抗性的机理和应用研究进展[J]. 湖北农业科学, 2010, 49(1):204−206. Wang W N, Quan X J, Xiao C G, et al. Research advances on application and mechanism of induced resistance of plant[J]. Hubei Agricultural Sciences, 2010, 49(1): 204−206.
[16]	Francis I, Holsters M, Vereecke D. The gram-positive side of plant-microbe interactions[J]. Environmental Microbiology, 2010, 12(1): 1−12. doi: 10.1111/j.1462-2920.2009.01989.x
[17]	黄晓丽. 毛壳菌(Chaetomium spp.)生防菌株的筛选及其生物防治机制研究[D]. 成都: 四川农业大学, 2009. Huang X L. Screens biocontrol strains from Chaetomium spp. and studies on biocontrol mechanisms of Chaetomium spp.[D]. Chengdu: Sichuan Agricultural University, 2009.
[18]	高珍娜, 吕文静, 谢航, 等. 纤维素降解菌的筛选与诱变育种[J]. 福州大学学报(自然科学版), 2010, 38(3):450−455. Gao Z N, Lü W J, Xie H, et al. Screening and breeding by induced mutation of cellulose-degrading strains[J]. Journal of Fuzhou University (Natural Science Edition), 2010, 38(3): 450−455.
[19]	周德庆. 微生物学教程[M]. 北京: 高等教育出版社, 2011: 385. Zhou D Q. Microbiology tutorial[M]. Beijing: Higher Education Press, 2011: 385.
[20]	王瑞芹. 油茶内生拮抗细菌Y13定殖动态及对叶内微生物调整效应研究[D]. 长沙: 中南林业科技大学, 2014. Wang R Q. Colonization and adjusted effect of inoculating with antagonistic strains Y13 on microbe in Camellia oleifera[D]. Changsha: Central South University of Forestry and Technology, 2014.
[21]	Li A L, Chen B J, Li G H, et al. Physalis alkekengi L. var. franchetii (Mast.) Makino: an ethnomedical, phytochemical and pharmacological review[J]. Journal of Ethnopharmacology, 2018, 210: 260−274.
[22]	Santos G C, Cardoso F P, Martins A D, et al. Polyploidy induction in Physalis alkekengi[J]. Bioscience Journal, 2020, 36(3): 827−835.
[23]	卢丽俐. 油茶炭疽病拮抗内生细菌的筛选、发酵及抑菌机理研究[D]. 长沙: 中南林业科技大学, 2009. Lu L L. Screening, fermentation conditions and inhibiting mechanisms of antagonistic strains against Camellia oleifera anthracnose caused by Colletotrichum gloeosporioides[D]. Changsha: Central South University of Forestry and Technology, 2009.
[24]	孟庆敏. 油茶炭疽病内生拮抗细菌Y13抑菌物质分离纯化及结构鉴定[D]. 长沙: 中南林业科技大学, 2014. Meng Q M. Isolation and identification of main antimicrobial substance produced by endophytic bacteria Y13 in the Camellia oleifera[D]. Changsha: Central South University of Forestry and Technology, 2014.
[25]	徐广春, 顾中言, 罗楚平, 等. 枯草芽孢杆菌生防菌剂sf-628专用助剂的研发[J]. 果树学报, 2012, 29(5):895−899. Xu G C, Gu Z Y, Luo C P, et al. Study on special adjuvants of antibiological inoculant Bacillus subtilis sf-628[J]. Journal of Fruit Science, 2012, 29(5): 895−899.
[26]	陆燕, 李澄, 陈志德, 等. 解淀粉芽孢杆菌41B-1对花生白绢病的生防效果[J]. 中国油料作物学报, 2016, 38(4):487−494. Li Y, Li C, Chen Z D, et al. Biological control activities of Bacillus amyloliquefaciens 41B-1 against Sclerotium rolfsii[J]. Chinese Journal of Oil Crop Sciences, 2016, 38(4): 487−494.
[27]	陈志谊, 刘永锋, 陆凡. 水稻纹枯病生防菌Bs-916产业化生产关键技术[J]. 植物保护学报, 2004(3):230−234. Chen Z Y, Liu Y F, Lu F. Key technology for industrial production of biocontrol bacteria Bs-916 against rice sheath blight[J]. Journal of Plant Protection, 2004(3): 230−234.
[28]	Rajkumar M, Lee Whlee K J. Screening of bacterial antagonists for biological control of Phytophthora blight of pepper[J]. Journal of Basic Microbiology, 2005, 45(1): 55. doi: 10.1002/jobm.200410445.
[29]	Jensen L G, Olsen O, Kops O, et al. Transgenic barley expressing a protein-engineered, thermostable (1,3-1,4)-beta-glucanase during germination[J]. Proceedings of the National Academy of Sciences of the United States of America, 1996, 93(8): 3487−3491. doi: 10.1073/pnas.93.8.3487
[30]	Denning D W. Echinocandin antifungal drugs[J]. Lancet, 2003, 362: 1142−1151. doi: 10.1016/S0140-6736(03)14472-8.

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Inhibition effects of biocontrol bacteria strains on the pathogen of Camellia oleifera anthracnose