Effects of different concentrations of IBA, NAA on rooting of etiolated shoots of <i>Quercus variabilis</i> and <i>Quercus mongolica</i>

Zhang Jie; Li Jiankang; Duan An’an; Sun Yuhan; Liu Hongshan; Wu Suran; Li Guifen; Li Yun

doi:10.13332/j.1000-1522.20190103

Journal of Beijing Forestry University > 2019 > 41(7): 128-138. > DOI: 10.13332/j.1000-1522.20190103

Zhang Jie, Li Jiankang, Duan An’an, Sun Yuhan, Liu Hongshan, Wu Suran, Li Guifen, Li Yun. Effects of different concentrations of IBA, NAA on rooting of etiolated shoots of Quercus variabilis and Quercus mongolica[J]. Journal of Beijing Forestry University, 2019, 41(7): 128-138. DOI: 10.13332/j.1000-1522.20190103

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Effects of different concentrations of IBA, NAA on rooting of etiolated shoots of Quercus variabilis and Quercus mongolica

1.
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
2.
College of Forestry, Southwest Forestry University, Kunming 650224, Yunnan, China
3.
Hongya Mountain State-Owned Forest Farm Administration Bureau of Hebei Province, Yixian 074200, Hebei, China

More Information

Received Date: February 27, 2019
Revised Date: June 03, 2019
Available Online: July 03, 2019
Published Date: June 30, 2019

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Abstract

Abstract

ObjectiveThe effects of different concentrations of NAA and IBA on the rooting of one-year-old semi-lignified etiolated shoots of the selected provenances of Quercus variabilis and Quercus mongolica were investigated, aiming to find out what kind of mass concentration is most favorable for the rooting of semi-lignification of the etiolated shoot cuttings of current-year seedlings from fine provenances of Quercus variabilis and Quercus mongolica, and to select the most suitable mass concentration, and provide technical support for the asexual selection of Quercus variabilis and Quercus mongolica.
MethodIn this study, semi-lignified etiolated shoots of current-year seedlings from selected provenances of Quercus variabilis and Quercus mongolica were used as materials. Two kinds of growth regulators（Butyric acid (IBA) and naphthylacetic acid (NAA)）were used. Setting 6 mass concentrations of 3 000, 2 000, 1 500, 750, 600, 400 mg/L, respectively, and rinsing, non-etiolated semi-lignified shoots of the seedlings were used as control to investigate the effects of different concentrations of IBA and NAA on rooting of etiolated shoots of Quercus variabilis and Quercus mongolica.
ResultThe results showed that the etiolated cuttings were superior to the non-etiolated cuttings in rooting rate, rooting number, root length and root diameter. There were significant differences in adventitious rooting induction between two plant growth regulators for shoot cuttings of Quercus variabilis and Quercus mongolica. When the concentration was 2 000, 1 500, 750, 600, 400 mg/L for IBA, the rooting effects of treated etiolated or non-etiolated shoots of Quercus variabilis and Quercus mongolica were better than those of NAA. Under each mass concentration of growth regulators, the rooting indexes such as the adventitious root incidence and the number of roots of etiolated Quercus variabilis and Quercus mongolica were significantly higher than those of the non-etiolated cuttings. Under the condition of 1 500 mg/L, rooting rate of the etiolated Quercus variabilis was 20.0% higher than that of the non-etiolated one, and the rooting rate of etiolated Quercus mongolica was 14.6% higher than that of the non-etiolated one.
Conclusion1 500 mg/L IBA is the best plant growth regulator treatment for the etiolated and non-etiolated semi-lignified shoots of the current-year seedlings of Quercus variabilis and Quercus mongolica from superior provenance. The rooting rate of adventitious roots and rooting number were the highest, among which the rooting rate of etiolated Quercus variabilis cuttings was 100%, and that of etiolated Quercus mongolica cuttings was 84.6%.
- Quercus variabilis,
- Quercus mongolica,
- etiolated shoot cuttings,
- plant growth regulator,
- adventitious rooting induction

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References

[1]	冯健. 我国栎类遗传育种研究进展[J]. 辽宁林业科技, 2015(1):43−47. doi: 10.3969/j.issn.1001-1714.2015.01.014 Feng J. Advances in Quercus genetics and breeding in China[J]. Liaoning Forestry Science and Technology, 2015(1): 43−47. doi: 10.3969/j.issn.1001-1714.2015.01.014
[2]	卢楠, 孟丙南, 孙宇涵, 等. 四倍体刺槐黄化嫩枝扦插生根过程生理生化分析[J]. 东北林业大学学报, 2013, 41(11):5−9. doi: 10.3969/j.issn.1000-5382.2013.11.002 Lu N, Meng B N, Sun Y H, et al. Physiology and biochemistry during etiolated shoots cutting of tetraploid Robinia pseudoacacia[J]. Journal of Northeast Forestry University, 2013, 41(11): 5−9. doi: 10.3969/j.issn.1000-5382.2013.11.002
[3]	裴东. 核桃不定根发生调控机制与蛋白组学探讨[D]. 北京: 北京林业大学, 2004. Pei D. Regulation mechanism and proteomics of adventitious roots in walnut[D]. Beijing: Beijing Forestry University, 2004.
[4]	Henrique A, Campinhos E N, Ono E O, et al. Technetium-99m ceftizoxime kit preparation[J]. Brazilian Archives of Biology and Technology, 2006, 49(2): 189−196.
[5]	丁彤. 北美红栎无性繁殖体系的研究[D]. 合肥: 安徽农业大学, 2012. Ding T. Study on asexual reproduction system of northern red oak[D]. Hefei: Anhui Agricultural University, 2012.
[6]	闫文涛, 佟兆庆, 魏俊, 等. 不同生长调节剂对蒙古栎嫩枝扦插生根的影响[J]. 西北林学院学报, 2017, 32(3):116−121. doi: 10.3969/j.issn.1001-7461.2017.03.22 Yan W T, Tong Z Q, Wei J, et al. Cuttage propagation technique and rooting mechanism of Quercus mongolica with softwood cutting[J]. Journal of Northwest Forestry University, 2017, 32(3): 116−121. doi: 10.3969/j.issn.1001-7461.2017.03.22
[7]	刘家财, 滕士元, 史骥清. 弗吉尼亚栎的引种与扦插繁殖生产试验研究[J]. 中国园艺文摘, 2011, 27(6):24−25. doi: 10.3969/j.issn.1672-0873.2011.06.010 Liu J C, Teng S Y, Shi J Q. Study on introduction and cuttage propagation of Quercus virginiana in Wujiang City, China[J]. Chinese Horticultural Abstracts, 2011, 27(6): 24−25. doi: 10.3969/j.issn.1672-0873.2011.06.010
[8]	吴幸连. 大叶栎扦插繁殖技术研究[J]. 防护林科技, 2008(5):25−26. doi: 10.3969/j.issn.1005-5215.2008.05.010 Wu X L. Cutting propagation of Castanopsis fissa[J]. Protective Forest Technology, 2008(5): 25−26. doi: 10.3969/j.issn.1005-5215.2008.05.010
[9]	吕秀立, 杨芳, 施季森, 等. 苏玛栎无性繁殖试验[J]. 上海交通大学学报(农业科学版), 2013, 31(3):58−62. doi: 10.3969/J.ISSN.1671-9964.2013.03.010 Lü X L, Yang F, Shi J S, et al. Asexual reproduction experiment of Quercus shumardii[J]. Journal of Shanghai Jiaotong University (Agricultural Science Edition), 2013, 31(3): 58−62. doi: 10.3969/J.ISSN.1671-9964.2013.03.010
[10]	胡婉仪, 涂炳冲. 栓皮栎、麻栎、小叶栎、苦槠、石栎扦插繁殖简报[J]. 湖北林业科技, 1992(2):35−36. Hu W Y, Tu B C. Brief report on cuttage propagation of Quercus variabilis, Quercus acutissima, Quercus chenil, Castanopsis sclerophylla, Lithocrpus glaber[J]. Forestry Science and Technology of Hubei, 1992(2): 35−36.
[11]	陈丽英, 祁树安. 激素种类、浓度及浸泡时间对红举扦插育苗的影响[J]. 山东农业科学, 2018, 50(8):72−76. Chen L Y, Qi S A. Influences of hormone types, concentrations and soaking time on cutting propagation of Zelkova schneideriana[J]. Shandong Agricultural Science, 2018, 50(8): 72−76.
[12]	故丽米热·卡克什, 木合塔尔·扎热, 马合木提·阿不来提, 等. 不同浓度IBA对大果榛子不同品种黄化萌条扦插成活率影响综合评价[J]. 新疆农业科学, 2018, 55(3):468−476. Kakix G, Zari M, Ablat M, et al. Comprehensive evaluation of different concentration IBA effects on the survival rate of yellow sprouted bud cuttings of different big fruit hazelnut cultivars[J]. Xinjiang Agricultural Sciences, 2018, 55(3): 468−476.
[13]	张乐华, 王书胜, 单文, 等. 基制、激素种类及其浓度对鹿角杜鹃扦插育苗的影响[J]. 林业科学, 2014, 50(3):45−54. doi: 10.3969/j.issn.1672-8246.2014.03.010 Zhang L H, Wang S S, Shan W, et al. Influences of growth media, and hormone types and concentrations on cutting propagation of Rhododendron latoucheae[J]. Scientia Silvae Sinicae, 2014, 50(3): 45−54. doi: 10.3969/j.issn.1672-8246.2014.03.010
[14]	Shi C, Liu Y, Hu C. Research on hardy branch cutting cultivation of Qinghai spruce[J]. Acta Agriculturae Universitis Jiangxiensis, 2002, 24(2): 259−263.
[15]	Husen A, Pal M. Metabolic changes during adventitious root primordium development in Tectona grandis, Linn. f. (teak) cuttings as affected by age of donor plants and auxin (IBA and NAA) treatment[J]. New Forests, 2007, 33(3): 309−323. doi: 10.1007/s11056-006-9030-7
[16]	郭素娟. 林木扦插生根的解剖学及生理学研究进展[J]. 北京林业大学学报, 1997, 19(4):64−69. doi: 10.3321/j.issn:1000-1522.1997.04.011 Guo S J. Progress of study on rooting anatomy and physiology of forest tree cuttings[J]. Journal of Beijing Forestry University, 1997, 19(4): 64−69. doi: 10.3321/j.issn:1000-1522.1997.04.011
[17]	王艳晶, 彭祚登. 不同生根促进剂对国槐嫩枝扦插生根过程中内源激素变化的影响[J]. 西北林学院学报, 2017, 32(5):109−114. doi: 10.3969/j.issn.1001-7461.2017.05.19 Wang Y J, Peng Z D. Effects of different rooting accelerators on changes of endogenous hormones in Sophora japonica during softwood-cutting rooting process[J]. Journal of Northwest Forestry University, 2017, 32(5): 109−114. doi: 10.3969/j.issn.1001-7461.2017.05.19
[18]	申展. 闽楠无性繁殖技术研究[D]. 长沙: 中南林业科技大学, 2013. Shen Z. Asexual propagation techniques of Phoebe bournei[D]. Changsha: Central South University of Forestry and Technology, 2013.
[19]	高茜茜, 刘苑秋, 郭圣茂, 等. 扦插环境及基质对晚松嫩枝扦插生根的影响[J]. 林业科技通讯, 2018(2):31−34. Gao Q Q, Liu Y Q, Guo S M, et al. Effects of cutting environment and substrate on rooting of Pinus rigida Mill. var. serotina cuttings[J]. Forestry Science and Technology Newsletter, 2018(2): 31−34.
[20]	裴东, 谷瑞升. 树木复幼的研究概述[J]. 植物学通报, 2005, 22(6):753−760. doi: 10.3969/j.issn.1674-3466.2005.06.016 Pei D, Gu R S. A review on the rejuvenation of mature trees[J]. Chinese Bulletin of Botany, 2005, 22(6): 753−760. doi: 10.3969/j.issn.1674-3466.2005.06.016

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[2]	YAN Shao-peng, SHANG Yan-ru, LENG Shu-jiao, YANG Rui-hua, WANG Qiu-yu. Gene expression profile of aspen hybrid cuttings in two developing stages of the adventitious root.[J]. Journal of Beijing Forestry University, 2015, 37(10): 9-13. DOI: 10.13332/j.1000-1522.20130230
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[4]	YANG Li-xue, WANG Hai-nan, FAN Jing. Effects of donor tree ages and plant growth regulators on the softwood cutting propagation of Hippophae rhamnoides[J]. Journal of Beijing Forestry University, 2011, 33(6): 107-111.
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[7]	XUE Kang, HE Qian, LI Ji-yue, QI Tao. Effects of plant growth regulator “Shifengle” on transpiring water-consumption of Rhus chinensis and Rhus typhina[J]. Journal of Beijing Forestry University, 2007, 29(2): 82-87.
[8]	HE Qian, LI Ji-yue, QI Tao. Influence of plant growth regulator on transpiring water consumption of Sophora japonica L.[J]. Journal of Beijing Forestry University, 2007, 29(1): 74-78. DOI: 10.13332/j.1000-1522.2007.01.013
[9]	SUN Xiao-mei, ZHANG Shou-gong, WANG Xiao-shan, QI Li-wang, LÜ Shou-fang, WANG Jian-hua. Effects of growth regulators on nursery rooting and seedling growth of hybrid larch[J]. Journal of Beijing Forestry University, 2006, 28(2): 68-72.
[10]	LI Jie, HUANG Min-ren, WANG Ming-xiu, CAI Ru. Effects of exogenous growth regulators on somatic embryogenesis of Cymbidium hybridum[J]. Journal of Beijing Forestry University, 2005, 27(4): 65-68.

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Effects of different concentrations of IBA, NAA on rooting of etiolated shoots of Quercus variabilis and Quercus mongolica