Genetic relationship analysis and molecular identification of <i>Magnolia wufengensis</i> cultivars based on SSR and SRAP markers

Zhang Mei; He Zhengquan; Ma Jiang; Sang Ziyang; Zhu Zhonglong; Zhang Dechun; Ma Lüyi; Chen Faju

doi:10.13332/j.1000-1522.20190204

Journal of Beijing Forestry University > 2019 > 41(9): 69-80. > DOI: 10.13332/j.1000-1522.20190204

Zhang Mei, He Zhengquan, Ma Jiang, Sang Ziyang, Zhu Zhonglong, Zhang Dechun, Ma Lüyi, Chen Faju. Genetic relationship analysis and molecular identification of Magnolia wufengensis cultivars based on SSR and SRAP markers[J]. Journal of Beijing Forestry University, 2019, 41(9): 69-80. DOI: 10.13332/j.1000-1522.20190204

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Genetic relationship analysis and molecular identification of Magnolia wufengensis cultivars based on SSR and SRAP markers

1.
Key Laboratory of Three Gorges Regional Plant Genetics & Germplasm Enhancement (CTGU), Hubei Engineering Research Center for Three Gorges Regional Plant Breeding Biotechnology Research Center, China Three Gorges University, Yichang 443000, Hubei, China
2.
Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China
3.
Forestry Research Institute of Wufeng County, Wufeng 443413, Hubei, China
4.
Wufeng Magnolia Technology Development Company Limited, Yichang 443400, Hubei, China

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Received Date: April 28, 2019
Revised Date: June 26, 2019
Available Online: July 09, 2019
Published Date: August 31, 2019

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Abstract

Abstract

ObjectiveGenetic diversity and genetic variation of Magnolia wufengensis cultivars were examined by simple sequence repeats (SSR) and sequence-related amplified polymorphism (SRAP) molecular markers for establishing its molecular marker system.
MethodThirty-five simples of M.wufengensis cultivars were applied to PCR amplification for SSR and SRAP markers. The genetic similarity coefficient of each cultivar was calculated by NTSYS-pc2.1 and cluster analysis was carried out using UPGMA.The locus data of combination of primer pairs that can discriminate all cultivars were screened by R language.
ResultA total of 18 pairs of SSR primers with polymorphism and clear bands were obtained. With the genome DNA of 35 M. wufengensis cultivars as the PCR template, a total of 128 polymorphic bands were generated. The number of polymorphic bands at each polymorphic primer ranged from 2 to 15, with mean band number of 7.1, mean pattern number of 10.6, mean effective pattern number of 5.4 and mean D of 0.72.The mean value of the percentage of polymorphic loci was 0.730 2, ranging from 0.142 9 ~ 1.000 0. The mean Shannon's index was 0.304 2, ranging from 0.086 4 to 0.433 7. The mean expected heterozygosity among cultivars was 0.248 8, ranging from 0.059 2 to 0.282 3.Eleven pairs of effective SRAP primers were acquired through screening. A total of 156 polymorphic bands were produced with the genome DNA of 35 M.wufengensis cultivars as the template. The number of polymorphic bands at each polymorphic primer ranged from 7 to 18, with mean band number of 14.2, mean pattern number of 22.7, mean effective pattern number of 13.2 and mean D of 0.93. The mean value of the percentage of polymorphic loci was 0.815 6, ranging from 0.657 1 ~ 1.000 0. The mean Shannon’s index was 0.375 9, ranging from 0.287 2 to 0.455 3. The mean expected heterozygosity among cultivars was 0.240 4, ranging from 0.180 2 to 0.311 6.
ConclusionM. wufengensis has relatively high genetic diversity. The analysis of molecular variation of both SSR and SRAP marker systems indicates that most genetic diversity is within M. wufengensis cultivars. The locus data of combination of primer pairs can discriminate all cultivars for identifying M. wufengensis cultivars efficiently and accurately.These results provide important references for the protection and breeding of M. wufengensis.
- Magnolia wufengensis,
- genetic diversity,
- cultivar identification,
- SSR molecular marker,
- SRAP molecular marker

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References

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Genetic relationship analysis and molecular identification of Magnolia wufengensis cultivars based on SSR and SRAP markers