• Scopus
  • Chinese Science Citation Database (CSCD)
  • A Guide to the Core Journal of China
  • CSTPCD
  • F5000 Frontrunner
  • RCCSE
Advanced search
ZHANG Min, HUANG Hua-hong, LIN Er-pei, ZHOU Hou-jun, WANG Ya-hui, TONG Zai-kang.. Library construction of cDNA and SSR analysis of partial ESTs for stem and leaf of Betula luminifera.[J]. Journal of Beijing Forestry University, 2012, 34(4): 58-61.
Citation: ZHANG Min, HUANG Hua-hong, LIN Er-pei, ZHOU Hou-jun, WANG Ya-hui, TONG Zai-kang.. Library construction of cDNA and SSR analysis of partial ESTs for stem and leaf of Betula luminifera.[J]. Journal of Beijing Forestry University, 2012, 34(4): 58-61.
shu

Library construction of cDNA and SSR analysis of partial ESTs for stem and leaf of Betula luminifera.

  • Nurturing Station for State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Linan,311300, P.R.China.

More Information
  • Received Date: December 31, 1899
  • Revised Date: December 31, 1899
  • Published Date: July 29, 2012
    Graphical Abstract
    • Abstract
  • A cDNA library of stem and leaf from Betula luminifera was constructed. The primary titer of cDNA library was about 1.5×106 pfu/mL, its recombinant efficiency reached 97.3%, and the size of insert DNA fragments ranged from 0.5 to 3.0 kb, with an average of 1.3 kb. The results indicated that it was a higherquality cDNA library, and could be used in gene cloning and gene expression profile analysis. Distribution and frequency of SSRs were analyzed in 224 nonredundant ESTs from B. luminifera cDNA library, using online searching software. The results showed that 60 SSRs distributed in 47 EST sequences, accounting for 26.80% of all ESTs. Dinucleotide would be the major repeat types, accounting for 70.00% of the total number of acquired SSRs. The trinucleotide and tetranucleotide repeats accounted for 28.30% and 1.70% respectively. This research might lay the foundation for designing the targeted ESTSSR primers and genetic diversity analysis by mining the information of EST-SSR loci in B. luminifera EST sequence data.
    • FullText(HTML)
    • References (0)
    • Related Articles

  • Related Articles

    [1]Xu Xinghua, Huang Qilun, Li Shanwen, Meng Xianwei, Li Zongtai, Qiao Yanhui, Dong Yufeng, Zhang Zhiyi. Creation and comprehensive evaluation of Populus deltoides germplasm resources[J]. Journal of Beijing Forestry University, 2024, 46(8): 79-86. DOI: 10.12171/j.1000-1522.20220101
    [2]Du Kang, Wang Jiahuan, Li Junheng, Li Chao, Zhang Pingdong, Kang Xiangyang. Genetic identification and evaluation of cold-resistant germplasm resources in Populus tomentosa[J]. Journal of Beijing Forestry University, 2023, 45(12): 59-67. DOI: 10.12171/j.1000-1522.20220363
    [3]Wang Lina, Wu Junwen, Dong Qiong, Shi Zhuogong, Hu Haocheng, Wu Danzi, Li Luping. Effects of tending and thinning on non-structural carbon and stoichiometric characteristics of Pinus yunnanensis[J]. Journal of Beijing Forestry University, 2021, 43(8): 70-82. DOI: 10.12171/j.1000-1522.20210115
    [4]Li Wei, Wang Pan, Qiqige, Zhang Qichang, Huang Bingjun, Xiao Zejun. Phenotypic diversity analysis of blueberry germplasm resources[J]. Journal of Beijing Forestry University, 2020, 42(2): 124-134. DOI: 10.12171/j.1000-1522.20190279
    [5]Zhang Yichi, Guo Sujuan, Sun Chuanhao. Effects of growth retardants on anatomy and non-structural carbohydrates of chestnut leaves[J]. Journal of Beijing Forestry University, 2020, 42(1): 46-53. DOI: 10.12171/j.1000-1522.20180437
    [6]MIAO Yu-bo, ZHU Xiao-mei, LI Zhi-juan, JIA Feng-ling, LI Wei. Genetic evaluation of breeding resources of Pinus sylvestris var. mongolica from different improved generations[J]. Journal of Beijing Forestry University, 2017, 39(12): 71-78. DOI: 10.13332/j.1000-1522.20170194
    [7]HU Jia-hang, JI Xiao-di Xiao-di, LI Feng-long, GUO Ming-hui. Assessment of embodied environmental impact on log wooden wall member.[J]. Journal of Beijing Forestry University, 2017, 39(6): 116-122. DOI: 10.13332/j.1000-1522.20160307
    [8]JI Meng-cheng, SHAN Xiao-bin, ZHANG Yin-li. Investigation on Clematis resources in Zhejiang Province[J]. Journal of Beijing Forestry University, 2008, 30(5): 66-72.
    [9]ZHENG Xiao-xian. Theory research of forest resource transformation[J]. Journal of Beijing Forestry University, 2007, 29(6): 185-189. DOI: 10.13332/j.1000-1522.2007.06.024
    [10]LIU Jin-fu, HONG Wei, LI Jun-qing. Characteristics of gaps renewal in Castanopsis kawakamii forests[J]. Journal of Beijing Forestry University, 2006, 28(3): 14-19.

  • Cited by

    Periodical cited type(2)

    1. 程世平，姚鹏强，耿喜宁，刘春洋，谢丽华. 高温诱导牡丹产生未减数花粉. 园艺学报. 2022(03): 581-589 .
    2. 李琳，刘德旺，田野，黄林芳. 激光共聚焦显微镜技术在中药研究中的应用. 中成药. 2017(05): 1038-1041 .

    Other cited types(2)

Catalog

    Get Citation
    PDF
    XML
    Article views (1618) PDF downloads (68) Cited by(4)
    Turn off MathJax
    Article Contents
    Abstract
    Related Articles

    Copyright © 2013 《北京林业大学学报》编辑部

    Address：North-Star Times Tower, No.8 Beichendong Road, Chaoyang District, Beijing, China China Pos：100083

    Tel：010-62337673 Email：bldxed@bjfu.edu.cn 京ICP备05066833号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return