Comparative proteomics of two <i>Populus</i> spp.(Section <i>Tacamahaca</i>) allotriploid derived by different types of 2n female gamete and their parents

Wang Yi; Qiu Tong; Han Qiang; Kang Xiangyang

doi:10.13332/j.1000-1522.20170425

Journal of Beijing Forestry University > 2018 > 40(5): 1-9. > DOI: 10.13332/j.1000-1522.20170425

Wang Yi, Qiu Tong, Han Qiang, Kang Xiangyang. Comparative proteomics of two Populus spp.(Section Tacamahaca) allotriploid derived by different types of 2n female gamete and their parents[J]. Journal of Beijing Forestry University, 2018, 40(5): 1-9. DOI: 10.13332/j.1000-1522.20170425

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Comparative proteomics of two Populus spp.(Section Tacamahaca) allotriploid derived by different types of 2n female gamete and their parents

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China

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Received Date: November 28, 2017
Revised Date: January 13, 2018
Published Date: April 30, 2018

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Abstract

Abstract

ObjectiveAlthough Populus allotriploid has a prominent vegetative growth advantages, the underlying molecular mechanisms have not yet been revealed and elucidated. This work was designed to investigate proteins differentially expressed in the two Populus allotriploid derived by different types of 2n female gamete and their parents. The results will provide the scientific foundation for Populus polyploidy selection and genetic improvement.
MethodThe iTRAQ proteomics approach was used in this study. The extracted proteins were digested using FASP method and identified by iTRAQ coupled with LC-MS/MS technology. Raw data were analyzed by Proteome Discoverer 1.3 search engine. Then the pathway analysis was conducted using GO and KEGG.
ResultA total of 1 472 proteins were identified and 202 proteins were detected as differentially expressed proteins. The ratio of differentially expressed proteins between FDR and SDR Populus allotriploid and the two parents varied from 2.0% to 10.1%. Compared with the female parent or male parent, the ratio of differentially expressed proteins in FDR Populus allotriploid was higher than in SDR Populus allotriploid. In particular, there was an expression level dominance bias toward the triploid progenitors. Further analysis showed that the differentially expressed proteins were significantly enriched in the pathways such as metabolic related, ribosomes, photosynthesis and response to stress.
ConclusionThe results indicated that plyploidization and hybridization could enhance photosynthesis, proteins synthetic and increased the resistance and adaptability of Populus polyploidy. All of these contribute to vegetative growth advantages in allotriploid plants.
- 2n female gamete,
- Populus allotriploid,
- proteomics,
- iTRAQ

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References

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Comparative proteomics of two Populus spp.(Section Tacamahaca) allotriploid derived by different types of 2n female gamete and their parents