Establishment of protein differential expression map of Tamarix hispida leaves under NaHCO₃ stress

ObjectiveIn order to study the mechanism of Tamarix hispida responsing to the salt stress, this study is committed to optimize the extraction method of total protein from the leaves of Tamarix hispida. The main purpose of this research is to explore the method of extracting total protein which is suitable for two-dimensional electrophoresis of Tamarix hispida leaves, and establish the differential protein expression map of Tamarix hispida leaves under NaHCO₃ stress.

MethodImproved Tris/TCA, improved Tris/TCA/PVP40, improved Tris/TCA/Acetone and plant protein extraction kit methods were used to study the optional extracting protein methods of Tamarix hispida leaves. Differential expression of proteins in Tamarix hispida leaves under NaHCO₃ stress were studied using two-dimensional electrophoresis. And the protein differential expression map of Tamarix hispida leaves under salt stress was established.

ResultThis research revealed that we can obtain the high purity of protein sample and the map of two-dimensional gel electrophoresis which has good separation and high resolution using Tris/TCA method. The two-dimensional gel electrophoresis maps of proteins which were treated with NaHCO₃ for 0 h and 12 h were analyzed by ImageMaster 2D Platinum 7.0 gel analysis software. Twenty-five differentially expressed proteins were detected, which were responded to salt stress and the changes of differentially expressed proteins were analyzed.

ConclusionThe results showed that the extraction of Tamarix hispida leaf protein by improved Tris/TCA method is suitable for two-dimensional electrophoresis system, and this method could be used to study the proteome of Tamarix hispida leaves. In this study, the differential protein expression map of Tamarix hispida leaves under NaHCO₃ stress was established, and this result would be the foundation of the molecular mechanism of Tamarix hispida with salt tolerance for further analysis.